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化学学报
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化学学报 ›› 2001, Vol. 59 ›› Issue (12): 2181-2185. 上一篇    下一篇

研究论文

多糖与生物探针相互作用机理的研究: 透明质酸与天青A的反应

万晖;欧阳炜;焦庆才;刘茜;周尚根;丁黎华   

  1. 南京大学生命科学学院药物生物技术国家重点实验室
  • 发布日期:2001-12-15

Study on the interaction between polysaccharides and biological probes: Hyaluronic acid and azur A

Wan Hui;Ou Yangwei;Jiao Qingcai;Liu Qian;Zhou Shanggen;Ding Lihua   

  • Published:2001-12-15

PDF

700

被引次数

15

应用光谱法研究秀明质酸(HA)与天青A(AA)的相互作用机理，考察了反应体系中pH和AA/HA摩尔比对相互作用的影响，测得AA-HA复合物吸收光谱产生蓝移效应所需的临界AA/HA摩尔比1.28×10^3，HA对AA的最大结合数3.22×10^3，实验值与理论值完全一致，对多糖与生物探针的相互作用机理提出了合理的解释。

关键词: 多糖, 生物探针, 透明质酸, 天青A, 相互作用, 反应机理

The interaction of hyaluronic aicd (HA) and azur A(AA) was studied by absorption spectra. The influence of pH and the molar ratio of AA/HA on the spectra was investigated. The critical molar ratio (1.28×10^3) necessary fro the hypsochromism of the absorption spectra of AA-HA complex and the maximum binding number (3.22×10^3) were obtained by spectrometry. The maximum binding number is consistent with the theoretical value.

Key words: POLYSACCHARIDE, HYALURONIC ACID, INTERACTIONS, REACTION MECHANISM

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