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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (10): 982-988. 上一篇    下一篇

研究论文

基于离子液体共溶剂效应的氯过氧化物酶催化氧化邻苯二胺的研究

聂艳艳1，蒋育澄*,1,2，胡满成1,2，李淑妮1,2，翟全国1,2   

  1. (1陕西师范大学化学与材料科学学院 西安 710062)
    (2陕西师范大学大分子科学陕西省重点实验室 西安 710062)
  • 投稿日期:2009-10-10 修回日期:2009-12-29 发布日期:2010-01-21
  • 通讯作者: 蒋育澄 E-mail:jyc@snnu.edu.cn
  • 基金资助:

    国家自然科学基金(No. 20876094)资助项目

Studies on the Oxidation of σ-Phenylenediamine Catalyzed by Chloroperoxidase in the Presence of an Ionic Liquid as Co-solvent

Nie Yanyan1 Jiang Yucheng*,1,2 Hu Mancheng1,2 Li Shuni1,2 Zhai Quanguo1,2   

  1. (1 School of Chemistry and Materials Science, Shaanxi Normal University, Xi an 710062)
    (2 Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi an 710062)
  • Received:2009-10-10 Revised:2009-12-29 Published:2010-01-21

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1664

被引次数

24 | 3

通过缓冲液-离子液体混合溶剂中氯过氧化物酶(CPO)催化氧化邻苯二胺(OPD)的产物结构和性能的表征, 证实此酶促反应的产物为2,3-二氨基吩嗪(DAP); OPD-H2O2-CPO反应体系有望用于荧光酶联免疫分析; 酶动力学分析表明以咪唑类离子液体(ILS)为共溶剂时, CPO对底物的亲和力及对底物识别的专一性都得以改善, 从而有效提高了产物收率; 酶促反应主要受CPO的稳定性及酶的用量等因素控制. 在最佳条件下, 产率可达81.16%.

关键词: 氯过氧化物酶, 邻苯二胺, 2,3-二氨基吩嗪, 离子液体, 酶动力学

The oxidation of σ-phenylenediamine (OPD) catalyzed by chloroperoxidase (CPO) in the presence of an ionic liquids (ILS) as co-solvent was studied in this work. Identifications by UV-Vis, FT-IR and 13C and 1H NMR spectrum show that the product is 2,3-diaminophenazine (DAP). This ensures the application of (OPD-H2O2-CPO) system in fluorescence enzyme-link immunoassay. The enzyme kinetic assay indicated that both the affinity and specificity of CPO to the substrate was improved in the buffer-ILS mixture, which was the main reason of the enhancement of product yield. This enzyme-catalyzed reaction was controlled mainly by the stability and consumption of CPO. The DAP yield was 81.16% under the optimum condition.

Key words: chloroperoxidase, σ-phenylenediamine, 2,3-diaminophenazine, ionic liquids, enzyme kinetic assay

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