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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (11): 1103-1108. 上一篇    下一篇

研究论文

沙蒿籽水溶性胶多糖ASPI-A的结构与免疫活性的初步研究

郝毓倩1，黄琳娟1，齐春会2，王仲孚*,1   

  1. (1西北大学生命科学学院 西北大学糖生物学与糖工程研究中心 西安 710069)
    (2军事医学科学院毒物药物研究所 北京 100850)
  • 投稿日期:2009-09-18 修回日期:2010-01-15 发布日期:2010-01-25
  • 通讯作者: 黄琳娟 E-mail:huanglinjuan2@163.com
  • 基金资助:

    国家“十一五”科技支撑计划项目（批准号：2006BAD06B00）和国家高技术研究发展计划（“863”计划）（批准号：2007AA091601;2007AA10Z338和）资助

Structure Elucidation and Preliminary Immunological Activity Studies of the Water-soluble Gum Polysaccharide ASPI-A Isolated from Artemisia sphaerocephala Krasch Seeds

Hao Yuqian1 Huang Linjuan1 Qi Chunhui2 Wang Zhongfu*,1   

  1. (1 Glycobiology and Glycotechnology Research Center, Life Science College, Northwest University, Xi'an 710069)
    (2 Institute of Pharmacology and Toxicology, The Academy of Military Medical Sciences, Beijing 100850)
  • Received:2009-09-18 Revised:2010-01-15 Published:2010-01-25

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被引次数

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从沙蒿籽中提取出水溶性胶多糖, 经柱色谱分离纯化得到一种中性多糖组分ASPI-A. 采用高效凝胶渗透色谱法(HPGPC)测定其为均一性多糖, 平均分子量为5.42×104 Da. 经IR, GC部分酸水解、甲基化分析等方法对该多糖的化学结构进行了表征. 结果表明, 该多糖由阿拉伯糖、甘露糖、葡萄糖及半乳糖组成, 其物质的量的比为1∶2.8∶4.9∶1.9. ASPI-A为多分支结构, 以(1→4)-β-Glc构成主链, 部分葡萄糖C6存在分支, 由甘露糖以-4)Man(1-连接在葡萄糖C6位, Glc(1-和Gal(1-连接在甘露糖C4位构成. 免疫活性实验结果表明, ASPI-A在10～50 μg•mL－1浓度范围内对Con A诱导的小鼠T淋巴细胞增殖反应具有促进作用.

关键词: 沙蒿籽, 多糖, 分离纯化, 结构, 免疫活性

Artemisia sphaerocephala Krasch is an important resource of northwest in China, and it has potential pharmaceutical value and environment protective function. Our purpose to the study of Artemisia seeds polysaccharide is to get more theory support to illustrate the structure-function relationship in molecule research. A water-soluble gum polysaccharide ASPI-A was isolated from Artemisia seeds with water extraction and column chromatography. ASPI-A was determined to be homogeneous with average molecular weight of 5.42×104 Da by high performance gel permeation chromatography (HPGPC). Monosaccharide analysis by gas chromatography (GC) showed that it was composed of arabinose, mannose, glucose and galactose at a molar ratio of 1∶2.8∶4.9∶1.9. Based on IR, GC, partial hydrolysis with acid, methylation and GC-MS analysis, ASPI-A was shown to be a highly branched polysaccharide with a backbone of β-1,4-linked-Glucose. The backbone was partially substituted at O6 of glucosyl residues with mannose, and the non-reducing end were Glc(1- and Gal(1- which were attached to O4 of mannose. The immunological activity assay revealed that ASPI-A 10～50 μg•mL－1 can enhance the Con A induced T lymphocyte proliferation of mice.

Key words: Artemisia sphaerocephala Krasch seeds, polysaccharide, isolation and purification, structure, immunological activity