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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (2): 137-141. 上一篇    下一篇

研究论文

抗癌药长春新碱及其与微管蛋白相互作用的电化学研究

于泳1,2, 胡劲波1, 尚军1, 李启隆1   

  1. 1. 北京师范大学化学系, 北京, 100875;
    2. 中国科学院力学研究所微重力实验室, 北京, 100080
  • 投稿日期:2003-03-23 修回日期:2003-07-14 发布日期:2014-01-26
  • 通讯作者: 胡劲波,E-mail:hujingbo@bnu.edu.cn;Tel:(010)62209398;Fax:(010)62200567 E-mail:hujingbo@bnu.edu.cn
  • 基金资助:
    国家自然科学基金(No.20275007)资助项目.

Study on the Electrochemical Behaviors of Vincristine andthe Interaction of Vincristine with Tubulin

YU Yong1,2, HU Jing-Bo1, SHANG Jun1, LI Qi-Long1   

  1. 1. Department of Chemistry, Beijing Normal University, Beijing 100875;
    2. National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080
  • Received:2003-03-23 Revised:2003-07-14 Published:2014-01-26

PDF

491

被引次数

45

在0.05 mol/L Tris, 0.15 mol/L NaCl溶液中,用吸附伏安法研究长春新碱(VCR),其峰电位在-1.68 V (vs.Ag/AgCl),峰电流与1.0×10-8～2.0×10-7 mol/L VCR浓度成正比,检测限为7.0×10-9 mol/L.用常规脉冲极谱法、线性扫描和循环伏安法等研究该体系的电化学行为.实验表明,电极还原过程为具有吸附特征的不可逆过程.VCR的吸附符合Frumkin吸附等温式.也研究了VCR与微管蛋白的相互作用.实验表明,VCR与微管蛋白形成一电活性的结合物,这一结合物具有吸附性,且还原过程也为不可逆过程.

关键词: 长春新碱, 伏安法, 吸附, 微管蛋白

A sensitive reduction peak of vincristine (VCR) is obtained by adsorptive stripping voltammetry in 0.05 mol/L Tris and 0.15 mol/L NaCl buffer solution. The peak potential is -1.68 V (vs.Ag/AgCl). The peak current is directly proportional to the concentration of VCR (1.0×10-8 ～2 0×10-7 mol/L), with a detection limit of 7.0×10-9 mol/L. The electrochemical behavior of this system was studied by the methods of normal pulse polarography, linear sweep and cyclic voltammetry. The reduction process is irreversible with adsorptive characteristics. The adsorption of VCR obeys the Frumkin isotherm. The electrochemical behavior of the binding of VCR to tubulin was studied by cyclic voltammetry. The results showed that the reaction of tubulin dimer with VCR formed an electrochemically active complex, and the reduction process of the complex is irreversible.

Key words: vincristine(VCR), voltammetry, adsorption, tubulin