石墨烯纳米孔的制备及λ-DNA穿孔初步研究

doi:10.6023/A13121208

化学学报 ›› 2014, Vol. 72 ›› Issue (3): 378-381.DOI: 10.6023/A13121208 上一篇下一篇

所属专题：石墨烯

研究通讯

石墨烯纳米孔的制备及λ-DNA穿孔初步研究

王跃^a, 余旭丰^b, 刘芸芸^c, 谢骁^d, 程秀兰^b, 黄少铭^c, 王志民^a

a 上海交通大学农业与生物学院上海 200240;
b 上海交通大学微电子学院上海 200240;
c 温州大学化学与材料工程学院温州 325027;
d 东南大学-FEI纳皮米中心南京 210096

投稿日期:2013-12-03 发布日期:2014-02-11
通讯作者: 王志民，E-mail：zmwang@sjtu.edu.cn E-mail:zmwang@sjtu.edu.cn
基金资助:
项目受国家“863”计划（No. 2012AA02A104）；自然科学基金（No. 51302037）；上海交通大学重大项目及创新团队培育基金（No. X198775）和上海市重点学科建设基金（No. B209）资助.

Fabrication of Graphene Nanopores and a Preliminary Study on λ-DNA Translocation

Wang Yue^a, Yu Xufeng^b, Liu Yunyun^c, Xie Xiao^d, Cheng Xiulan^b, Huang Shaoming^c, Wang Zhimin^a

a School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240;
b School of Microelectronics, Shanghai Jiaotong University, Shanghai 200240;
c College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325027;
d SEU-FEI Nano-Pico Center, Southeast University, Nanjing 210096

Received:2013-12-03 Published:2014-02-11
Supported by:
Project supported by the National High Technology Research and Development Program of China (No. 2012AA02A104), the National Natural Science Foundation of China (No. 51302037), the Innovative Research Team fund of Shanghai Jiaotong University (No. X198775) and the Shanghai Key Discipline fund (No. B209).

文章分享

1. 石墨烯纳米孔的制备及λ-DNA穿孔初步研究.PDF(255KB)

摘要/Abstract

纳米孔测序是有可能实现“$1，000 Genome”目标的技术之一. 近年来，研究较多的纳米孔有蛋白质纳米孔和硅基材料的固态纳米孔. 蛋白孔寿命比较短，而基于硅基底的固态纳米孔深度显著超过单链DNA相邻碱基的间距，所以，无法实现DNA的单个碱基的分辨. 作者用聚焦离子束先制造氮化硅基底，并在该基底上铺设石墨烯，再用聚焦电子束刻蚀石墨烯，获得直径10 nm以下的纳米孔，初步分析了DNA穿越纳米孔时产生的电信号及穿孔噪音，向单层石墨烯纳米孔测序DNA迈出了一步.

关键词: 石墨烯, 纳米孔, 测序, 聚焦离子束, 聚焦电子束

Nanopore sequencing is among the most promising technologies to achieve the goals of the "$1,000 Genome". Two major types of nanopores have been extensively investigated, protein and silicon-based solid-state nanopores. However, protein pores are short-lived and the length of solid-state nanopores is much larger than the distance between adjacent bases, resulting in incapability to discriminate individual bases along the single-stranded DNA molecules. In this paper, we report λ-DNA translocations through graphene nanopore. A large nanopore with diameter of about 30 nm on silicon nitride substrates were first fabricated using focused ion beam (FIB) system under the beam current of 2 pA, accelerating voltage of 30 kV and sculpting time of 1 s. Individual graphene membranes were suspended onto the substrates to cover the large pore, and nanopores with a diameter less than 10 nm are sculpted in the graphene sheet by focused electron beam (FEB) from a transmission electron microscope (TEM) under a 400 kx magnification times and 300 kV accelerating voltage at 1×10⁵～5×10⁵ A/m²current density for 2～3 min. The edges of these graphene nanopores became smoother and sharper when the temperature was increased to about 450 ℃, which might help to lower interactions between graphene nanopores and the analytes. The signals of DNA translocation through graphene nanopores had been recorded using a patch clamp amplifier at 10 kHz sampling frequency filtered at 5 kHz via an integrated four-pole low-pass Bessel filter. Analyses of the DNA translocation current traces indicated that different conformations of DNA molecules may exist during entrance into nanopores. In addition, our overall detection platform had a low noise amplitude of around 10 pA, which allowed more sensitive signal detection. Taken together, our observations demonstrate that graphene nanopores are feasible for DNA sensing, leading a forward step towards single-molecule DNA sequencing using monolayered graphene nanopores.

Key words: graphene, nanopore, sequencing, focused ion beam, focused electron beam

引用此文

王跃, 余旭丰, 刘芸芸, 谢骁, 程秀兰, 黄少铭, 王志民. 石墨烯纳米孔的制备及λ-DNA穿孔初步研究[J]. 化学学报, 2014, 72(3): 378-381.

Wang Yue, Yu Xufeng, Liu Yunyun, Xie Xiao, Cheng Xiulan, Huang Shaoming, Wang Zhimin. Fabrication of Graphene Nanopores and a Preliminary Study on λ-DNA Translocation[J]. Acta Chimica Sinica, 2014, 72(3): 378-381.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

参考文献

[1] Bayley, H. Curr. Opin. Chem. Biol. 2006, 10, 628.

[2] Meyerson, M.; Gabriel, S.; Getz, G. Nat. Rev. Genet. 2010, 11, 685.

[3] Hayden, E. C. Nature 2008, 451, 378.

[4] Kayser, M.; de Knijff, P. Nat. Rev. Genet. 2011, 12, 179.

[5] Zhou, X.; Ren, L.; Li, Y.; Zhang, M.; Yu, Y.; Yu, J. Sci. China, Life Sci. 2010, 53, 44.

[6] Wanunu, M. Phys. Life Rev. 2012, 9, 125.

[7] Church, G.; Deamer, D. W.; Branton, D.; Baldarelli, R.; Kasianowicz, J. US 5795782, 1998 [Chem. Abstr. 1996, 126, 4207].

[8] Kasianowicz, J. J.; Brandin, E.; Branton, D.; Deamer, D. W. Proc. Natl. Acad. Sci. U. S. A. 1996, 93, 13770.

[9] Schneider, G. F.; Dekker, C. Nat. Biotech. 2012, 30, 326.

[10] Venkatesan, B. M.; Bashir, R. Nat. Nanotech. 2011, 6, 615.

[11] Deamer, D. W.; Akeson, M. Trends Biotech. 2000, 18, 147.

[12] Li, J.; Stein, D.; McMullan, C.; Branton, D.; Aziz, M. J.; Golovchenko, J. A. Nature 2001, 412, 166.

[13] Storm, A. J.; Chen, J. H.; Ling, X. S.; Zandbergen, H. W.; Dekker, C. Nat. Mater. 2003, 2, 537.

[14] Siwy, Z.; Fuliński, A. Phys. Rev. Lett. 2002, 89, 198103.

[15] Langecker, M.; Arnaut, V.; Martin, T. G.; List, J.; Renner, S.; Mayer, M.; Dietz, H.; Simmel, F. C. Science 2012, 338, 932.

[16] Liu, H.; He, J.; Tang, J.; Liu, H.; Pang, P.; Cao, D.; Krstic, P.; Joseph, S.; Lindsay, S.; Nuckolls, C. Science 2010, 327, 64.

[17] Wang, Z.; Huang, S. CN ZL200610147236. 3, 2006[Chem. Abstr. 2007, 147, 230108].

[18] Sha, J.; Hasan, T.; Milana, S.; Bertulli, C.; Bell, N. A. W.; Privitera, G.; Ni, Z.; Chen, Y.; Bonaccorso, F.; Ferrari, A. C.; Keyser, U. F.; Huang, Y. Y. S. ACS Nano 2013, 7, 8857.

[19] Liu, S.; Lu, B.; Zhao, Q.; Li, J.; Gao, T.; Chen, Y.; Zhang, Y.; Liu, Z.; Fan, Z.; Yang, F.; You, L.; Yu, D. Adv. Mater. 2013, 25, 4549.

[20] Meller, A.; Nivon, L.; Brandin, E.; Golovchenko, J.; Branton, D. Proc. Natl. Acad. Sci. U. S. A. 2000, 97, 1079.

[21] Venta, K.; Shemer, G.; Puster, M.; Rodriguez-Manzo, J. A.; Balan, A.; Rosenstein, J. K.; Shepard, K.; Drndi?, M. ACS Nano 2013, 7, 4629.

[22] Ying, Y.-L.; Zhang, J.; Gao, R.; Long, Y.-T. Angew. Chem., Int. Ed. 2013, 52, 13154.

[23] Ying, Y.; Zhang, X.; Liu, Y.; Xue, M.; Li, H.; Long, Y. Acta Chim. Sinica 2013, 71, 44. (应佚伦, 张星, 刘钰, 薛梦竹, 李洪林, 龙亿涛, 化学学报, 2013, 71, 44.)

[24] Venta, K.; Wanunu, M.; Drndi?, M. Nano Lett. 2013, 13, 423.

[25] Liu, A.; Zhao, Q.; Guan, X. Anal. Chim. Acta 2010, 675, 106.

[26] Garaj, S.; Hubbard, W.; Reina, A.; Kong, J.; Branton, D.; Golovchenko, J. Nature 2010, 467, 190.

[27] Merchant, C. A.; Healy, K.; Wanunu, M.; Ray, V.; Peterman, N.; Bartel, J.; Fischbein, M. D.; Venta, K.; Luo, Z.; Johnson, A. T. C.; Drndi?, M. Nano Lett. 2010, 10, 2915.

[28] Nelson, T.; Zhang, B.; Prezhdo, O. V. Nano Lett. 2010, 10, 3237.

[29] Postma, H. W. Nano Lett. 2010, 10, 420.

[30] Schneider, G. F.; Kowalczyk, S. W.; Calado, V. E.; Pandraud, G.; Zandbergen, H. W.; Vandersypen, L. M.; Dekker, C. Nano Lett. 2010, 10, 3163.

[31] Kim, M. J.; McNally, B.; Murata, K.; Meller, A. Nanotechnology 2007, 18, 205302.

[32] Liu, Q.; Wu, H.; Wu, L.; Xie, X.; Kong, J.; Ye, X.; Liu, L. Plos One 2012, 7, e46014.

[33] Wanunu, M.; Sutin, J.; McNally, B.; Chow, A.; Meller, A. Biophys. J. 2008, 95, 4716.

[34] Fologea, D.; Gershow, M.; Ledden, B.; McNabb, D. S.; Golovchenko, J. A.; Li, J. Nano Lett. 2005, 5, 1905.

[35] Tabard-Cossa, V.; Trivedi, D.; Wiggin, M.; Jetha, N. N.; Marziali, A. Nanotechnology 2007, 18, 305505.

[36] Clarke, J.; Wu, H.-C.; Jayasinghe, L.; Patel, A.; Reid, S.; Bayley, H. Nat. Nanotech. 2009, 4, 265.

[37] Li, J.; Gershow, M.; Stein, D.; Brandin, E.; Golovchenko, J. A. Nat. Mater. 2003, 2, 611.

[38] Smeets, R. M. M.; Keyser, U. F.; Dekker, N. H.; Dekker, C. Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 417.

[39] Hoogerheide, D. P.; Garaj, S.; Golovchenko, J. A. Phys. Rev. Lett. 2009, 102, 256804.

[40] Bayley, H. Nature 2010, 467, 164.

石墨烯纳米孔的制备及λ-DNA穿孔初步研究

Fabrication of Graphene Nanopores and a Preliminary Study on λ-DNA Translocation

PDF

补充材料

可视化

摘要/Abstract

引用此文

分享此文

参考文献

相关文章 15

编辑推荐

相关统计

本文评价

[1]	倪雪, 辛凯莉, 胡正利, 蒋翠玲, 万永菁, 应佚伦, 龙亿涛. 一种时序信号分类算法在纳米孔道离子电流信号识别中的应用^★[J]. 化学学报, 2023, 81(8): 912-919.
[2]	马超凡, 徐伟, 刘巍, 徐昌晖, 沙菁㛃. 受限纳尺度下蛋白质输运的主动操纵技术[J]. 化学学报, 2023, 81(7): 857-868.
[3]	宁聪聪, 杨倩, 毛阿敏, 唐梓嘉, 金燕, 胡宝山. 石墨烯纳米带的可控制备研究进展[J]. 化学学报, 2023, 81(4): 406-419.
[4]	刘稳, 王昱捷, 杨慧琴, 李成杰, 吴娜, 颜洋. 离子液体非共价诱导制备碳纳米管/石墨烯集流体用于钠金属负极[J]. 化学学报, 2023, 81(10): 1379-1386.
[5]	闫绍兵, 焦龙, 何传新, 江海龙. ZIF-67/石墨烯复合物衍生的氮掺杂碳限域Co纳米颗粒用于高效电催化氧还原[J]. 化学学报, 2022, 80(8): 1084-1090.
[6]	王旭生, 杨胥, 陈春辉, 李红芳, 黄远标, 曹荣. 石墨烯量子点/铁基金属-有机骨架复合材料高效光催化二氧化碳还原^※[J]. 化学学报, 2022, 80(1): 22-28.
[7]	翟耀, 辛国祥, 王佳琦, 张邦文, 宋金玲, 刘晓旭. 微波辅助合成具有优异电化学性能的rGO/CeO₂超级电容器电极材料[J]. 化学学报, 2021, 79(9): 1129-1137.
[8]	刘长安, 洪士博, 李蓓. 石墨烯在甘油/尿素剥离液中的稳定行为的分子动力学模拟研究[J]. 化学学报, 2021, 79(4): 530-538.
[9]	黄杰, 奚江波, 陈伟, 柏正武. 石墨烯衍生物作为无金属碳基催化剂在有机催化中的应用[J]. 化学学报, 2021, 79(11): 1360-1371.
[10]	岳华, 马光辉. 基于石墨烯独特生物界面效应的功能化载体研究进展[J]. 化学学报, 2021, 79(10): 1244-1256.
[11]	马明昊, 徐明, 刘思金. 氧化石墨烯的表面化学修饰及纳米-生物界面作用机理[J]. 化学学报, 2020, 78(9): 877-887.
[12]	李海梅, 罗华健, 肖琦, 杨立云, 黄珊, 刘义. 手性石墨烯量子点与DNA相互作用及其机制研究[J]. 化学学报, 2020, 78(6): 577-586.
[13]	赵雅婧, 谢亮, 马兰超, 贺军辉. 聚二甲基硅氧烷封装石墨烯基柔性红外探测器的制备及其应用[J]. 化学学报, 2020, 78(2): 161-169.
[14]	宋光捷, 武调弟, 刘福鑫, 张彬雁, 刘秀辉. 壳聚糖/氮掺杂还原氧化石墨烯修饰电极对黄嘌呤的检测及尿酸抑制的研究[J]. 化学学报, 2020, 78(1): 82-88.
[15]	郭宇, 李燕瑞, 王成名, 龙冉, 熊宇杰. TiO₂/石墨烯复合材料的光生电荷分离调控与光催化产氢性能研究[J]. 化学学报, 2019, 77(6): 520-524.