In this paper chitoson, controlled pore glass and silica-gel have been investigated with respect to its potentiality of being used as solid support of flow-injection immunoassay. A new flow-injection immunoassay end-point detection method has also been developed on the basis of coupling the reaction of H~2O~2 and K~4Fe(CN)~6, catalyzed by HRP, with H~2O~2 and K~3Fe(CN)~6 co-oxidized chemiluminescence reaction of luminol. As enzymatic and chemiluminescent reaction were generated at different sites of the detection system. The general shortcomings of such as unable to select a optimum pH for both enzymatic and chemiluminescent reaction, incomplete contact between enzyme and substrate, and scattering of support have been eliminated. It has the advantages of high sensitivity and good accuracy. HRP and its conjugates at f mol level can be detected within 1-2 min. and the relative standard deviation is 3.9%.

Key words: COUPLING REACTION, CHEMILUMINESSENCE, FLOW INJECTION ANALYSIS, LABELLED COMPOUNDS