A novel amperomatric biosensor has been developed. Glucose oxidase was successfully immobilized on a type β zeolite, and for the first time applied to the construction of the glucose biosensor based on employing TCNQ as a mediator. Such zeolite dispesion offers enhanced sensitivity, extended linear range and higher stability. Such improvements are attributed to the formation of a porous paste network that exposes the enzyme contained in the interior of the paste to the substrate solution and imparts higher mass transport resistance. Cyclic voltammetry and amperometric measurements demonstrated that TCNQ coimmobilized with glucose oxidase in this way displayed good stability and efficiently shuttled electrons between immobilized GOD and the ellectrode. The biosensor responds rapidly to glucose in the linear range from 5.0×10^-^6 to 2.0×10^-^3 mol/L for optimum analytical performance.

Key words: METHANE P, INFRARED SPECTROPHOTOMETRY, ZEOLITE, FOURIER TRANSFORM, BIOSENSORS, GLUCOSE OXIDASE, QUINONE P