The cellulose tris(4-methylbenzoate) (CTMB)-coated silanized gel was slurry-packed in stainless steel columns. the complete resolution of racemic 2-(dimethylamino)-2-phenylbutyl ester of 3, 4, 5- trimethoxybenzoic acid by CTMB-CSP (CSP=Chiral Stationary Phase has been achieved. Effects of the 2-propanol content in mobile phase and different temperature on capacity factor, separation factor, resolution in the separation of 2-(dimethylamino)-2-phenylbutyl ester of 3, 4, 5-trimethoxybenzoic acid enantiomers were studied. The thermodynamic parameters ΔΔGⅲ, ΔΔHⅲ, and ΔΔSⅲ have been estimated by the separation factor (α) at a given temperature, where the terms ΔΔGⅲ, ΔΔHⅲ, and ΔΔSⅲ represent the differences in the total free energy, enthalpy, and entropy differences, respectively. It was found that CTMB-CSP is sensitive to small changes in temperature. The resolution increased when the temperature was decreased in the 24.9℃ to 45.0℃ range.

Key words: CHIRALITY, STATIONARY PHASE, HIGH SPEED LIQUID CHROMATOGRAPHY, ENANTIOMORPH, ISOPROPANOL, CELLULOSE ESTERS, SEPARATION, DRUGS