The chemical components of volatile oil from Foemiculum Vulgare Mill were determined by GC-MS. Anethole accounted for 82.65% of the whole oil. Selective isolation of anethole form the volatile oil was carried out using an inclusion crystalline method with 1,1,6,6- tetraphenylhexa- 2,4-diyne-1,6-diol inclusion complex with anethole . The crystal structure of the inclusion complex was determined by IR, 1^H NMR and single crystal XRD techniques. The host-guest molar ratio in complex was found to be 2:1. Crystal structure of inclusion compound belong to space group P1, a=1.2972 (2) nm, b=1.5119(2)nm, c=1.6799(2)nm, α=100.57(1)°,β=104.89(1)°,γ=113.58(1)°,Z=2. The isolated chemical component was isolated from the inclusion crystal complex by using Kugelrohr vacuum distillation technique and was identified to by trans-anethole by means of IR, 1^H NMR , and MS spectroscopy. The results of GC analysis showed that chemical purity of trans-anethole isolated from volatile oil was 97% and the yield was 75.28%.

Key words: OLECULAR RECOGNITION, CRYSTALLIZE, SEPARATION, CHROMATOGRAPHY-MASS SPECTROGRAPHY