Hydrogen Peroxide Biosensor Based on Enzyme Multilayers through Electrostatic Adsorption

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (5): 502-507. Previous Articles Next Articles

基于静电吸附多层膜固定酶的过氧化氢生物传感器的研究

阳明辉, 李春香, 杨云慧, 沈国励, 俞汝勤

湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙, 410082

投稿日期:2003-07-17 修回日期:2003-11-11 发布日期:2014-02-18
通讯作者: 沈国励,E-mail:glshen@hnu.cn E-mail:glshen@hnu.cn
基金资助:
国家自然科学基金(Nos.20075006,29975006)、博士点基金(No.20010532008)和湖南省自然科学基金(Nos.01JJY1003,01JJY2099)资助项目.

Hydrogen Peroxide Biosensor Based on Enzyme Multilayers through Electrostatic Adsorption

YANG Ming-Hui, LI Chun-Xiang, YANG Yun-Hui, SHEN Guo-Li, YU Ru-Qin

State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082

Received:2003-07-17 Revised:2003-11-11 Published:2014-02-18

Abstract

A hydrogen peroxide biosensor has been prepared, and the procedure is as follows: firstly 2,6-pyridinedicarboxylic acid is electropolymerized onto a glassy carbon electrode to form a negatively charged surface; secondly, the positively charged polyelectrolyte poly(allylamine hydrochloride) (PAH) layer is constructed by the self-assembly technique to immobilize the negatively charged horseradish peroxidase through electrostatic adsorption; lastly, the multi-enzyme layers are fixed by the above methods and the biosensor are constructed. The effects of experimental conditions such as applied potential, mediator concentration and pH values on the electrode performance have been investigated, and the reproducibility, interferences and lifetime of the electrode are also tested. The linear response of detection of the electrode to hydrogen peroxide is in the concentration range of 4.6×10^-6～3.5×10^-3 mol/L with a detection limit of 2×10 ^-6 mol/L. The electrode was applied to the determination of recoveries of real sample with satisfactory results.

Key words: 2, 6-pyridinedicarboxylic acid, polyelectrolyte, horseradish peroxidase, biosensor

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YANG Ming-Hui, LI Chun-Xiang, YANG Yun-Hui, SHEN Guo-Li, YU Ru-Qin. Hydrogen Peroxide Biosensor Based on Enzyme Multilayers through Electrostatic Adsorption[J]. Acta Chimica Sinica, 2004, 62(5): 502-507.

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基于静电吸附多层膜固定酶的过氧化氢生物传感器的研究

Hydrogen Peroxide Biosensor Based on Enzyme Multilayers through Electrostatic Adsorption

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