Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (22): 2069-2076. Previous Articles     Next Articles

Original Articles

DNA与金属锇配合物相互作用的表面电化学研究

张志凌,左超,庞代文*   

  1. (武汉大学化学与分子科学学院 武汉 430072)
  • 投稿日期:2005-02-18 修回日期:2005-08-10 发布日期:2010-12-10
  • 通讯作者: 庞代文

Study of the Interaction between DNA and Osmium Complexes by Surface Electrochemical Method

ZHANG Zhi-Ling, ZUO Chao, PANG Dai-Wen*   

  1. (College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
  • Received:2005-02-18 Revised:2005-08-10 Published:2010-12-10
  • Contact: PANG Dai-Wen

The interaction of dsDNA and ssDNA with two osmium complexes has been studied by surface electrochemical methods based on DNA modified electrode to find that both osmium complexes could bind DNA with electrostatic interaction even at high ionic strength. And some thermodynamic and kinetics constants, such as binding constant K3+ or K2+, ratio of binding constants K3+/K2+, limiting ratio at zero ionic strength (Ii=0), free energy ΔGb, dissociation rate constant k and binding site size s (base pair) have been obtained.

Key words: DNA, osmium complex, interaction, surface electrochemistry