Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (24): 2857-2861. Previous Articles     Next Articles

Special Topic

层层自组装法制备碳纳米管/酶多层膜电极及其检测酚类物质

杨绍明1,黄爱花2,魏志鹏2,郑龙珍2   

  • 投稿日期:2009-03-30 修回日期:2009-07-23 发布日期:2010-02-04
  • 通讯作者: 杨绍明 E-mail:yangsm@yahoo.cn

CNT/enzyme Multilayer Electrode Prepared by Layer-by-Layer self-Assembly and Its Detection for Phenols

  • Received:2009-03-30 Revised:2009-07-23 Published:2010-02-04

A horseradish peroxidase (HRP) multilayer enzyme electrode was constructed with carbon
nanotubes (CNT) dispersed by sodium dodecylsulfonate (SDS) and HRP via layer-by-layer (LBL)
self-assembly. UV-Vis spectroscopy and SEM were used to monitor the uniform LBL self-assembly of CNT
and HRP. With catechol as substrate, electrochemical study showed that the use of CNT could obviously
improve the sensitivity of the HRP electrode, and the response of the electrode increased with the increment
of the CNT/HRP bilayer. The performances of the (CNT/HRP)4 electrode were studied for the phenols determination.
The experimental results showed a wide linear range, good sensitivity and anti-interference
ability when the HRP electrode was applied to the determination of phenols (catechol, hydroquinone and
phenol).

Key words: carbon nanotube, horseradish peroxidase, phenol, layer-by-layer self-assembly