Surface Enhanced Raman Spectroscopic Studies on Immunoassay by 4,4'-Bipyridyl as Readout Molecule

Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (3): 240-244. Previous Articles Next Articles

表面增强拉曼光谱研究以4,4'-联吡啶为标记分子的免疫检测

蒋芸，崔颜，姚建林，顾仁敖*

(苏州大学化学系苏州 215006)

投稿日期:2005-03-22 修回日期:2005-10-25 发布日期:2006-02-14
通讯作者: 顾仁敖

Surface Enhanced Raman Spectroscopic Studies on Immunoassay by 4,4'-Bipyridyl as Readout Molecule

JIANG Yun, CUI Yan, YAO Jian-Lin, GU Ren-Ao*

(Department of Chemistry, Suzhou University, Suzhou 215006)

Received:2005-03-22 Revised:2005-10-25 Published:2006-02-14
Contact: GU Ren-Ao

Abstract

The adsorption behaviour of reporter molecules (4,4'-bipyridyl (4,4'-bipy)) was investigated on gold nanoparticles by surface enhanced Raman spectroscopy (SERS). The immunogold nanoparticles with SERS reporters were prepared by the immobilization of goat antimouse IgG onto 4,4'-bipy modified gold nanoparticles, and the goat antimouse IgG was immobilized on a glass slide to obtain solid antibody. The sandwich structure of antibody/antigen/reporter-labelled immunogold nanoparticles was built by assembling solid antibody and SERS immunogold with corresponding antigen. The single or multiple type biospecific species could be identified through the detection on the SERS signal of the reporters coimmobilized with immunogold nanoparticles.

Key words: surface enhanced Raman spectroscopy, gold nanoparticle, IgG, 4,4'-bipyridyl, immunoassay

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JIANG Yun, CUI Yan, YAO Jian-Lin, GU Ren-Ao*. Surface Enhanced Raman Spectroscopic Studies on Immunoassay by 4,4'-Bipyridyl as Readout Molecule[J]. Acta Chimica Sinica, 2006, 64(3): 240-244.

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表面增强拉曼光谱研究以4,4'-联吡啶为标记分子的免疫检测

Surface Enhanced Raman Spectroscopic Studies on Immunoassay by 4,4'-Bipyridyl as Readout Molecule

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