Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (17): 2039-2046. Previous Articles     Next Articles

Full Papers

基于CdTe量子点测定烟酸诺氟沙星的新方法研究

魏宏,王云云,宋尔群   

  1. (发光与实时分析教育部重点实验室 西南大学药学院 重庆 400715)
  • 投稿日期:2010-12-14 修回日期:2011-04-25 发布日期:2011-05-19
  • 通讯作者: 宋尔群 E-mail:eqsong@swu.edu.cn
  • 基金资助:

    国家自然科学基金;中央高校基本科研业务费;重庆市自然科学基金资助项目;西南大学博士基金

New Method for Detection of Norfloxacin Nicotinate Based on the CdTe Quantum Dots

WEI Hong, WANG Yun-Yun, SONG Er-Qun   

  1. (Key Laboratory of Luminescence and Real-Time Analysis of the Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715)
  • Received:2010-12-14 Revised:2011-04-25 Published:2011-05-19

The fluorescence quenching of norfloxacin nicotinate to thioglycolic acid stabilized CdTe quantum dots (QDs) has been systematically investigated by using CdTe QDs as a fluorescence probe. On the basis of that, a sensitive method for the determination of norfloxacin nicotinate was developed. The linear equation was DF=26.6206c-20.8273 (μg/mL) and its linear range was 0.10~90 μg/mL with correlation coefficient of 0.997. The limit of detection was 2.50×10-2 μg/mL. Then the proposed new method was applied to measure the quantity of real sample (such as norfloxacin nicotinate solid powder), compared with the labeled amount, the accuracy was within 93.0%~104.0% and the RSD value for every sample five times determinations was or lower than 4.2%. This new method shows a bright application prospect for detection of norfloxacin nicotinate due to its several advantages such as accurate, high sensitivity, and wide detection range while compared with traditional ultraviolet spectrophotometry.

Key words: quantum dot, norfloxacin nicotinate, fluorescence quenching

CLC Number: