Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging

doi:10.6023/A12070461

Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (20): 2137-2143.DOI: 10.6023/A12070461 Previous Articles Next Articles

Article

寡聚芴纳米粒子的制备、表征与细胞成像应用

温泉山, 唐红伟, 杨高买, 刘礼兵, 吕凤婷, 杨琼, 王树

中国科学院化学研究所有机固体实验室北京 100190

投稿日期:2012-07-24 发布日期:2012-09-11
通讯作者: 刘礼兵, 王树 E-mail:wangshu@iccas.ac.cn; liulibing@iccas.ac.cn
基金资助:
项目受国家自然科学基金(Nos. 21033010, 21021091)和国家重点基础研究发展计划(Nos. 2011CB932302, 2011CB808400)资助.

Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging

Wen Quanshan, Tang Hongwei, Yang Gaomai, Liu Libing, Lv Fengting, Yang Qiong, Wang Shu

Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190

Received:2012-07-24 Published:2012-09-11
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21033010, 21021091) and the Major Research Plan of China (Nos. 2011CB932302, 2011CB808400).

A new hydrophobic oligofluorene (OF) is synthesized via Suzuki cross-coupling reaction. The absorption and fluorescence spectra are measured in chloroform with high molar absorption coefficient (1.08?10⁵ mol^-1稬穋m^-1) and fluorescence quantum yield (0.96). OF is dispersed into water to form nanoparticles with average size of 230 nm from dynamic light scattering (DLS) measurement. The nanoparticles in water still possess high molar absorption coefficient and fluorescence quantum yield. The quantitative cytotoxicity of OF nanoparticles to human lung cancer (A549) cells is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, showing that OF nanoparticles exhibit low cellular toxicity. OF nanoparticles can be utilized for imaging of live A549 cells. Confocal laser scanning microscopy demonstrates that OF nanoparticles are exclusively located in the cytoplasm, especially around the perinuclear region. OF nanoparticles exhibit good overlap staining with lysosome-specific dye (Lyso Tracker Red), which demonstrates that OF nanoparticles could be used for lysosome-specific imaging.

Key words: conjugated oligomer, nanoparticles, cellular imaging, lysosome

Cite this article

Wen Quanshan, Tang Hongwei, Yang Gaomai, Liu Libing, Lv Fengting, Yang Qiong, Wang Shu. Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging[J]. Acta Chimica Sinica, 2012, 70(20): 2137-2143.

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寡聚芴纳米粒子的制备、表征与细胞成像应用

Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging

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