Electrodeposition of Gold Nanoparticle Clusters on Multi-Wall Carbon Nanotubes and the Direct Electrochemistry of Hemoglobin

doi:10.6023/A12110877

Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (02): 239-245.DOI: 10.6023/A12110877 Previous Articles Next Articles

Article

三维金纳米团簇/多壁碳纳米管-Nafion膜修饰电极的电化学制备及血红蛋白的直接电化学

赵越^a, 洪波^a, 范楼珍^b

a 中国海洋大学环境科学与工程学院青岛 266100;
b 北京师范大学化学学院北京 100875

投稿日期:2012-11-06 发布日期:2013-01-09
通讯作者: 赵越 E-mail:yuezhao@ouc.edu.cn
基金资助:
国家自然基金(No. 41201569/D011102)和中央高校基本科研业务费实验室研究基金项目(No. 201251007).

Electrodeposition of Gold Nanoparticle Clusters on Multi-Wall Carbon Nanotubes and the Direct Electrochemistry of Hemoglobin

Zhao Yue^a, Hong Bo^a, Fan Louzhen^b

a College of Environmental Science and Technology, Ocean University of China, Qingdao 266100;
b College of Chemistry, Beijing Normal University, Beijing 100875

Received:2012-11-06 Published:2013-01-09
Supported by:
Project supported by the National Natural Science Foundation of China (No. 41201569/D011102) and the Fundamental Research Funds for the Central Universities (No. 201251007).

1. Electrodeposition of Gold Nanoparticle Clusters on Multi-Wall Carbon Nanotubes and the Direct Electrochemistry of Hemoglobin.PDF(181KB)

Abstract

Three-dimensional structure gold nanoparticle clusters (3D Au) were electrodeposited onto multiwalled carbon nanotubes (MWCNTs) through improved three-step method, which involves: (1) potential cycling (CV, 200 mV·s^-1) from +1.8 to -0.4 V was performed in 0.5 mol/L K₂SO₄ solutions for 10 min in order to produce oxide functional groups (carbonyl, hydroxyl, and carboxyl) at the defect sites located at the ends and/or the sidewalls of MWCNTs, (2) electrochemical oxidation of the Au(III) complex to the Au(V) complex from 2 mol/L K₂PtCl₄+0.1 mol/L K₂SO₄ aqueous solutions by using potential-step method (PS). The potential was jumped from 0.3 V to 1.1 V with different pulse width and this was repeated until a steady pulse current was reached. and (3) electrochemical transformation of the Au(V) complex to Au nanoparticle clusters on the surface of MWCNTs through cycling from +1.0 to -0.26 V in 0.1 mol/L H₂SO₄ solutions to the steady state. For comparison, however, experiments were also carried out with CV in the second step (2 mol/L K₂PtCl₄+0.1 mol/L K₂SO₄ aqueous solution with 190 cycles: these proved to be the optimal conditions). The morphology of 3D Au/MWCNTs electrode was characterized by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS). The voltammetric behavior of hemoglobin (Hb) on 3D Au/MWCNTs-Nafion has been investigated in 10 nmol/L Hb solution (pH=6 PBS) by cyclic voltammetry (CV) and current-time method (CT). Compared with Au+MWCNTs-Nafion (+means mix together), Au/MWCNTs-Nafion (uniform Au nanoparticle dispersion of MWCNTs, which was obtained when the second step adopts cyclic voltammetry instead of potential-step method), 3D Au/MWCNTs-Nafion gave a higher peak current and better reversibility. The higher sensitivity and lower detection limit show that 3D Au/MWCNTs-Nafion can offer a conductive microenvironment for the immobilized Hb to achieve direct electrochemistry. The average coverage (Γ) of Hb immobilized on 3D Au/MWCNTs-Nafion was calculated to be 7.65×10^-9 mol·cm^-2, testifies a high surface-to-volume ratio. Electron transfer rate constant (k_S) was calculated to be 1.8 s^-1, which was proved 3D Au/MWCNTs-Nafion is more conducive to direct electron transfer between Hb and the electrode. Hb/Au/MWCNTs-Nafion potential application towards the electrocatalytic reduction of H₂O₂ was also conducted to show immobilized Hb exhibits excellent biocompatibility and stability. Our work points to a new path for the preparation of 3D Au/MWCNTs nanocomposites, which are promising as electrocatalysts in direct electrochemistry of Hb.

Key words: three dimensional, gold nanoparticle, multi-wall carbon nanotubes, hemoglobin, direct electrochemistry

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Zhao Yue, Hong Bo, Fan Louzhen. Electrodeposition of Gold Nanoparticle Clusters on Multi-Wall Carbon Nanotubes and the Direct Electrochemistry of Hemoglobin[J]. Acta Chimica Sinica, 2013, 71(02): 239-245.

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三维金纳米团簇/多壁碳纳米管-Nafion膜修饰电极的电化学制备及血红蛋白的直接电化学

Electrodeposition of Gold Nanoparticle Clusters on Multi-Wall Carbon Nanotubes and the Direct Electrochemistry of Hemoglobin

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