有机化学 ›› 2015, Vol. 35 ›› Issue (4): 789-801.DOI: 10.6023/cjoc201409036 上一篇    下一篇

综述与进展

基于激发态能量转移机理比率型荧光探针的研究进展

陈忠林a, 李红玲a, 韦驾a, 肖义b, 于海波a   

  1. a 辽宁大学环境学院 沈阳 110036;
    b 大连理工大学 精细化工国家重点实验室 大连 116024
  • 收稿日期:2014-09-23 修回日期:2014-11-18 发布日期:2014-12-02
  • 通讯作者: 于海波 E-mail:yuhaibo@lnu.edu.cn
  • 基金资助:

    国家自然科学基金(No. 21302080)、辽宁省教育厅科研(No. L2014010)资助项目.

Recent Progress in Ratiometric Fluorescent Probes Based on Excitation Energy Transfer Mechanism

Chen Zhonglina, Li Honglinga, Wei Jiaa, Xiao Yib, Yu Haiboa   

  1. a College of Environmental Sciences, Liaoning University, Shenyang 110036;
    b State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024
  • Received:2014-09-23 Revised:2014-11-18 Published:2014-12-02
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21302080), and the Program Funded by Liaoning Province Education Administration (No. L2014010).

激发态能量转移(Excitation Energy Transfer, EET)作为一类重要的光物理现象, 被广泛用于比率型荧光探针和分子灯标的设计以及DNA检测等多个领域. 影响EET效率的两个重要因素是供受体间的空间距离和光谱交盖, 通过调节供受体间的空间距离或光谱重叠程度来调控能量转移过程, 实现对目标客体的双波长比率检测. 综述了基于不同供受体荧光团的EET体系、供受体间的连接方式对能量转移效率的影响, 以及通过调控供受体间光谱重叠程度或空间距离, 获得识别不同客体的比率型荧光探针, 并对EET机理的比率型荧光探针的设计以及未来在生物成像和医学检测等领域的应用进行了展望.

关键词: 荧光探针, 激发态能量转移, Fö, rster能量转移, 比率型荧光探针, 荧光发色团

Excitation energy transfer (EET) is one of the vital photophysical phenomenons, which is widely used in many applications, such as the design of ratiometric fluroesent probes, molecular beacon, DNA analysis, and so on. The process of energy transfer from donor to acceptor can be regulated by two factors: the spatial distance between donor and acceptor, and the spectral overlaps between donor emission and acceptor absorption, which results that there is a wide variety in the ratio at two different wavelengths of ratiometric fluorescent probes. In this review, noticeable EET systems with different donor fluorophore, connection form and energy transfer efficiency between donor and acceptor, and the modulation of spatial distance or spectral overlap are summarized. Finally, as a promising tool, the future developing prospects of EET fluorescent probes in bioimaging and medical diagnostics are discussed and highlighted.

Key words: fluorescent probe, excitation energy transfer, Fö, rster resonance energy transfer, ratiometric probe, fluorophore