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有机化学
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有机化学 ›› 1999, Vol. 19 ›› Issue (6): 582-586. 上一篇    下一篇

研究论文

蛋氨酸合成酶辅酶模型的合成及其催化反应的初步研究

刘俊义;吴晓华;王凤荣   

  1. 北京医科大学生物有机化学系;唐山市药品检验所
  • 发布日期:1999-12-25

Study on synthesis and catalytic mechanism of coenzyme model of methionine synthase

Liu Junyi;Wu Xiaohua;Wang Fengrong   

  • Published:1999-12-25

被引次数

2 | 1

生物体内的蛋氨酸合成酶(methioninesynthase)以N^5-甲基四氢叶酸作为辅酶,通过两步SN2反应,将甲基转移到高半胱氨酸的巯基上生成蛋氨酸。该酶起到中间甲基载体的功能。为了进一步研究甲基转移的机理,报道了作为活化的5-甲基四氢叶酸模型:碘化2-氨基-4-羟基-5,5,6-三甲基吡啶并[3,2-d]嘧啶的合成以及与模拟蛋氨酸合成酶[Co(I)(dmgH)~2Py]的反应。

关键词: 甲基转移, 嘧啶P, 催化机理, 合成, 吡啶并, 甲基转移酶, 蛋氨酸, 合成酶, 辅酶

As a model compound in methyl transfer process from N^5-methyltetra- hydrofolate(III) to methionine synthase, 2-amino-4-hydroxy-5, 5, 6- trimethyl-5, 6, 7, 8-tetrahydropyrido [3, 2-d] pyrimidinium iodide 8 was synthesized from 5-aminouacil through seven stepsof reactions. Compound 8 reacts with [Co(I)(dmgH)~2Py] to give CH~3-Co(dmgH)~2Py. The structures of all compounds were characterized by elemental analysis, UV, ^1H NMR and MS.

Key words: SYNTHESIS, SYNTHETASE, PYRIDINO-, CATALYTIC MECHANISM, COENZYME, PYRIMIDINE P, METHYL TRANSFERASE, METHIONINE

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