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有机化学
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有机化学 ›› 2008, Vol. 28 ›› Issue (03): 489-493. 上一篇    下一篇

研究简报

一种含光亲和标记异戊烯侧链探针的合成与初步表征

李令东a,b，唐伟a,b，刘武军a,b，赵宗保*,a   

  1. (a中国科学院大连化学物理研究所生物技术部 大连 116023)
    (b中国科学院研究生院 北京 100049)
  • 收稿日期:2007-07-05 修回日期:2007-08-20 发布日期:2008-03-18
  • 通讯作者: 赵宗保

Synthesis and Activity of the Photoaffinity-Labeled Functional Probe Based on Prenyl Side-Chain

LI Ling-Donga,b,TANG Weia,b,LIU Wu-Juna,b,ZHAO Zong-Bao*,a   

  1. (a Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023)
    (b Graduate School of Chinese Academy of Sciences, Beijing 100049)
  • Received:2007-07-05 Revised:2007-08-20 Published:2008-03-18
  • Contact: ZHAO Zong-Bao

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1135

被引次数

3

经5步反应、以9%的总收率合成了一种含生物素修饰和光亲和标记的异戊烯侧链功能探针分子, 初步考察了反应条件下该探针分子与Saccharomyces cerevisiae AS 2.399粗蛋白的相互作用; 生物素印迹分析结果表明, 酵母中多种蛋白被探针分子修饰, 为进一步开展化学蛋白组学研究奠定了基础.

关键词: 异戊稀侧链, 功能探针, 光亲和标记, 化学蛋白组学

A novel functional probe bearing both a benzophenone moiety and a biotin moiety was synthesized in 5 steps with an overall yield of 9%. Photoaffinity labeling and biotin blot experiment with the probe and the crude protein extracts from Saccharomyces cerevisiae AS 2.399 revealed that the synthetic probe was attached to a number of proteins, providing a basis for further chemical proteomics research.

Key words: functional probe, photoaffinity labeling, prenyl side-chain, chemical proteomics