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有机化学
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有机化学 ›› 2010, Vol. 30 ›› Issue (01): 66-71. 上一篇    下一篇

研究论文

含萘磺酰基N3O2大环化合物与DNA相互作用的光谱法研究

陈稼轩，田怡，向清祥*，张丽群，熊俊如   

  1. (乐山师范学院化学与生命科学学院 乐山 614004)
  • 收稿日期:2009-03-25 修回日期:2009-06-25 发布日期:2010-01-20
  • 通讯作者: 向清祥 E-mail:qxxiang711@163.com; xqxiang711@yahoo.com.cn
  • 基金资助:

    四川高校重点实验室专项（NO. 2006ZD049）;四川省教育厅（NO. 07ZC023）;乐山师范学院（NO. Z07045）项目

Spectroscopy Studies of Interactions between DNA and N3O2-Donor Macrocycle Bearing Naphathalic Sulfonic Group

Chen,Jiaxuan，Tian,Yi，Xiang,Qingxiang*，Zhang,Liqun，Xiong,Junru   

  1. (Department of Chemistry and Life Science，Leshan Teachers College，Leshan 614004)
  • Received:2009-03-25 Revised:2009-06-25 Published:2010-01-20

PDF

1105

被引次数

10

以8,11,14-三氮杂-5,6:16,17-二苯并-1,4-二氧杂环十七烷为原料，合成了含萘磺酰基的N3O2大环化合物，其结构经1H NMR，MS，元素分析等表征。通过紫外光谱法和荧光光谱法研究了N3O2大环化合物与小牛胸腺DNA的作用机制。结果表明: N3O2大环化合物通过嵌插方式与DNA结合，结合常数为 ＝20706.9 dm3•mol－1， ＝12416.9 dm3•mol－1，二者结合为焓驱动反应。

关键词: N3O2大环化合物, 合成, 小牛胸腺DNA, 光谱

A N3O2-donor macrocycle bearing a naphathalic sulfonic group derived from 8,11,14-triaza-5,6: 16,17-dibenzo-1,4-dioxacycloheptadecane has been synthesized，and its structure was characterized by 1H NMR，MS data and elemental analysis。The interactions of the target compound with CT (calf thymus) DNA have been investigated by the ultraviolet spectrophotometry and fluorescence spectrophotometry。The results showed that the binding model between the N3O2-donor macrocycle and CT DNA was intercalation，with the binding constants being ＝20706.9 dm3•mol－1 and ＝12416.9 dm3•mol－1。The com-bination was the enthalpy driven reaction.

Key words: N3O2-donor macrocycle, synthesis, CT DNA, spectrum