有机化学 ›› 2018, Vol. 38 ›› Issue (3): 612-628.DOI: 10.6023/cjoc201709020 上一篇    下一篇

所属专题: 荧光探针-生物传感合辑 有机超分子化学合辑

综述与进展

活性氧簇的小分子荧光探针研究进展

后际挺a,b, 李坤c, 覃彩芹a,b, 余孝其c   

  1. a 湖北工程学院 生物质资源转化利用湖北省协同创新中心 孝感 432000;
    b 湖北工程学院化学与材料科学学院 孝感 432000;
    c 四川大学化学学院 成都 610064
  • 收稿日期:2017-09-13 修回日期:2017-10-17 发布日期:2017-10-31
  • 通讯作者: 覃彩芹,E-mail:qincq@hbeu.edu.cn E-mail:qincq@hbeu.edu.cn
  • 基金资助:

    国家自然科学基金(No.31371750)资助项目.

Review of the Small Molecular Fluorescent Sensors for Intracellular Reactive Oxygen Species

Hou Jitinga,b, Li Kunc, Qin Caiqina,b, Yu Xiaoqic   

  1. a Hubei Collaboration Innovation Center for Biomass Conversion and Utilization, Hubei Engineering University, Xiaogan 432000;
    b School of Chemistry and Materials Science, Hubei Engineering University, Xiaogan 432000;
    c College of Chemistry, Sichuan University, Chengdu 610064
  • Received:2017-09-13 Revised:2017-10-17 Published:2017-10-31
  • Contact: 10.6023/cjoc201709020 E-mail:qincq@hbeu.edu.cn
  • Supported by:

    Project supported by the National Natrual Science Foundation of China (No. 31371750).

在参与体内各种化学反应的生物活性分子中,活性氧(reactive oxygen species,ROS)是一类非常重要的物质,它们具有氧化性,能够维持细胞内的氧化还原平衡,与细胞的生长或凋亡息息相关.因此,对于细胞内活性氧簇的荧光分析一直以来都受到广大研究人员的密切关注.然而由于ROS本身自有的一些特性,如存在寿命短、反应活性高等,该类荧光分析始终面临着一些难题,如选择性较低、副反应较多.总结了近十几年来细胞中活性氧的荧光识别与检测的研究进展,着重介绍了各类荧光探针的设计机理与生物应用,并对未来该类探针的发展进行了展望.

关键词: 活性氧簇, 荧光探针, 细胞成像

Among the numerous bio-active species which are involved in the various chemical reactions in our body, reactive oxygen species (ROS) are a class of important biological species, which are oxidative. ROS can maintain the intracellular redox balance and are tightly related with the cell growth and death. Hence, the fluorescence detection of intracellular ROS has attracted wide attention. However, owining to the inherent features of ROS, such as the short lifetime and high reactivity, fluorescence analysis of ROS is always faced with some problems, like low selectivity and side reactions. Herein, the development of small molecular fluorescent probes for intracellular ROS over the past decade is summarized, and the design mechanisms and bio-applications of these probes are emphasized.

Key words: reactive oxygen species (ROS), fluorescent sensor, intracellular imaging