有机化学 ›› 2022, Vol. 42 ›› Issue (8): 2428-2432.DOI: 10.6023/cjoc202203023 上一篇    下一篇

研究论文

一种检测ONOO的新型荧光探针的构建与细胞成像研究

李嘉欣, 贺如艳, 段森林, 黎锦华, 韩校净, 叶勇*()   

  1. 郑州大学化学学院 郑州 450001
  • 收稿日期:2022-03-12 修回日期:2022-04-21 发布日期:2022-05-26
  • 通讯作者: 叶勇
  • 作者简介:
    共同第一作者
  • 基金资助:
    郑州大学大学生创新创业训练计划(2021cxcy215); 郑州大学本科生拔尖计划资助项目.

Construction and Cell Imaging Study of a Novel Fluorescent Probe for ONOO Detection

Jiaxin Li, Ruyan He, Senlin Duan, Jinhua Li, Xiaojing Han, Yong Ye()   

  1. College of Chemistry, Zhengzhou University, Zhengzhou 450001
  • Received:2022-03-12 Revised:2022-04-21 Published:2022-05-26
  • Contact: Yong Ye
  • About author:
    These authors contributed equally to this work.
  • Supported by:
    Innovation and Entrepreneurship Training Plan for College Students of Zhengzhou University(2021cxcy215); Top Undergraduates Program of Zhengzhou University

过氧亚硝酸盐(ONOO)作为一种重要的活性氮, 与多种疾病的发生密切相关, 发展检测ONOO的方法对于理解其相关生理病理机制具有重要的意义. 以四苯乙烯作为荧光团, 引入吡喃鎓基团作为ONOO的特异识别位点, 成功构建一例新型的ONOO荧光探针TPE-ONOO. 该探针对ONOO具有较好的选择性、较强的抗干扰能力以及较高的灵敏度. 细胞试验表明探针具有较低的细胞毒性, 并成功将其用于细胞外源性和内源性ONOO的成像监测. 该新型探针为研究ONOO生理过程提供了一种有效的研究工具.

关键词: 荧光探针, ONOO, 四苯乙烯, 细胞成像

As an important reactive nitrogen species, peroxynitrite (ONOO) is closely related to the occurrence of various diseases. The development of methods to detect ONOO is of great significance for understanding its related physiological and pathological mechanisms. A novel ONOO fluorescent probe TPE-ONOO was successfully constructed using tetraphenylethylene as a fluorophore and a pyrylium group as the specific recognition site of ONOO. The probe showed high selectivity, strong anti-interference ability and high sensitivity to ONOO. Cell assays suggested that the probe had low cytotoxicity and was successfully used for imaging monitoring of exogenous and endogenous ONOO in cells. The novel probe synthesized here provides an efficient tool for studying ONOOphysiological processes.

Key words: fluorescent probe, ONOO, tetraphenylethylene, cell imaging