Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (9): 2485-2491.DOI: 10.6023/cjoc201903010 Previous Articles     Next Articles

Special Issue: 荧光探针-生物传感合辑 有机超分子化学合辑



常永新a, 李白a, 郭淼b, 蔡永红a, 徐括喜a   

  1. a 河南大学化学化工学院 功能有机分子研究中心 河南省阻燃与功能材料工程实验室 开封 475004;
    b 河南大学纳米材料工程研究中心 开封 475004
  • 收稿日期:2019-03-05 修回日期:2019-03-18 发布日期:2019-04-11
  • 通讯作者: 蔡永红, 徐括喜;
  • 基金资助:


A Novel Logic Gate Fluorescent Sensor for the Sequential Detection of Cd2+ and Pyrophosphate Anion and Application in Cell Imaging

Chang Yongxina, Li Baia, Guo Miaob, Cai Yonghonga, Xu Kuoxia   

  1. a Engineering Laboratory for Flame Retardant and Functional Materials of Henan Province, Institute of Functional Organic Molecular Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004;
    b Institute of Nanomaterials Engineering Research Center, Henan University, Kaifeng 475004
  • Received:2019-03-05 Revised:2019-03-18 Published:2019-04-11
  • Contact: 10.6023/cjoc201903010;
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. U1404207).

A novel fluorescent sensor L based on 8-hydroxyquinoline (8-HQ) has been designed and synthesized. The sensor L displayed excellent sensitivity specific toward Cd2+ and pyrophosphate anion (PPi), respectively. The fluorescence emission peak at 537 nm was quenched when Cd2+ was added to the DMSO/H2O (V/V=1/1, 0.01 mol/L, Hepes-HCl buffer, pH=7.20) solution of sensor L and the detection limit was as low as 5.87×10-8 mol/L. The 1:1 stoichiometry was verified by Job's plot and mass spectrum, and the association constant was obtained to be 4.37×104 L/mol. The further study found that the L-Cd2+ system has high selectivity for detection of PPi by ligand replacement method and L could be used as a logic gate fluorescence sensor. In addition, the sensor L was employed for imaging the Cd2+ and PPi in living cells.

Key words: 8-hydroxyquinoline, fluorescent sensor, sequential detection, cell imaging