Synthesis of Anticogulant Warfarin and Its Derivatives by the Crude Earthwarm Extract

doi:10.6023/cjoc201612035

Chin. J. Org. Chem. ›› 2017, Vol. 37 ›› Issue (6): 1494-1500.DOI: 10.6023/cjoc201612035 Previous Articles Next Articles

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蚯蚓催化抗凝血药华法林及其衍生物的合成

李志林^a, 周海燕^b, 官智^a

a. 西南大学化学化工学院重庆市应用化学重点实验室重庆 400715;
b. 西南大学生命科学学院重庆 400715

收稿日期:2016-12-11 修回日期:2017-01-11 发布日期:2017-02-08
通讯作者: 官智 E-mail:guanzhi@swu.edu.cn
基金资助:
“国家级大学生创新创业训练计划”基金（No.201510635004）资助项目.

Synthesis of Anticogulant Warfarin and Its Derivatives by the Crude Earthwarm Extract

Li Zhilin^a, Zhou Haiyan^b, Guan Zhi^a

a. Key Laboratory of Applied Chemistry of Chongqing Municipality, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715;
b. School of Life Sciences, Southwest University, Chongqing 400715

Received:2016-12-11 Revised:2017-01-11 Published:2017-02-08
Contact: 10.6023/cjoc201612035 E-mail:guanzhi@swu.edu.cn
Supported by:
Project supported by the National Training Program of Innovation and Entrepreneurship for Undergraduates (No. 201510635004).

1. Synthesis of Anticogulant Warfarin and Its Derivatives by the Crude Earthwarm Extract.PDF(8174KB)

Abstract

This article describes the synthesis of warfarin and its derivatives by using the crude earthwarm extract as a biocatalyst. Warfarin, an effective anticogulant being used for half a century, has enormous clinic and commercial value. Warfarin and its derivatives are usually prepared through chemical catalysis while biocatalysis was rarely explored. Because biocatalysis is green, sustainable and usually under mild conditions, it is necessary to develop biocatalytic methods for these compounds. It is found that the crude extract of earthwarms is a fine biocatalyst. Earthwarms are harmless and abundant in nature. There are many hydrolases in the alimentary tract of earthwarms, and among of them, proteases are most thoroughly studied. The crude earthwarm extract from Eisenia foetida was prepared, and it was used as a cheap and ecologically friendly biocatalyst for the synthesis of warfarin and its derivatives. The procedure is simple and no additional cofactors and equipments are required. The best results were obtained using l00 mg of earthworm extract in DMSO/water (10% water, V/V) with 1:3 molar ratio of 4-hydroxycoumarin to benzylideneacetone at 50 ℃ for 48 h. Warfarin was obtained in an excellent yield of 98%, and its derivatives were achieved in yields of 57%~99% with some enantioselectivities (up to 20% ee). In addition, control experiments demonstrated that enzyme(s) in the crude extract of earthwarms catalyzed Michael addition reaction. This work provides an alternative method for the synthesis of warfarin and its derivatives by using an easily available natural catalyst.

Key words: biocatalysis, the crude extract of earthworms, warfarin, Michael reaction

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Li Zhilin, Zhou Haiyan, Guan Zhi. Synthesis of Anticogulant Warfarin and Its Derivatives by the Crude Earthwarm Extract[J]. Chin. J. Org. Chem., 2017, 37(6): 1494-1500.

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蚯蚓催化抗凝血药华法林及其衍生物的合成

Synthesis of Anticogulant Warfarin and Its Derivatives by the Crude Earthwarm Extract

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