Chinese Journal of Organic Chemistry ›› 2022, Vol. 42 ›› Issue (3): 732-741.DOI: 10.6023/cjoc202108052 Previous Articles     Next Articles



李可欣a, 杨庆远a,*(), 张鹏鹏b, 张武元b,*()   

  1. a 西安交通大学化学工程与技术学院 西安 710049
    b 中国科学院天津工业生物技术研究所 天津 300308
  • 收稿日期:2021-08-28 修回日期:2021-09-30 发布日期:2021-10-21
  • 通讯作者: 杨庆远, 张武元
  • 基金资助:

Research Progress of Peroxygenase-Catalyzed Reactions Driven by in-situ Generation of H2O2

Kexin Lia, Qingyuan Yanga(), Pengpeng Zhangb, Wuyuan Zhangb()   

  1. a School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049
    b Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308
  • Received:2021-08-28 Revised:2021-09-30 Published:2021-10-21
  • Contact: Qingyuan Yang, Wuyuan Zhang
  • Supported by:
    National Natural Science Foundation of China(32171253)

Selective catalytic oxygen functionalization reactions of C—H bonds are a very challenging class of reactions in organic chemistry. Compared with tranditional metal complex catalysts, enzymes exhibit certain advantages in terms of selectivity and activity. Amongest them, peroxygenases are able to catalyze the oxidative functionalization of C—H bonds by direct use of H2O2 as a co-substrate. Peroxygenases combine the catalytic versatility of P450 monooxygenases without relying on coenzymes and their regenerative systems for cofactors. Therefore, peroxygenases have attracted considerable attention in organic synthesis. However, a bottleneck in the practical application of peroxygenase is that, like all heme-dependent enzymes, peroxygenase is sensitive to H2O2, and higher concentration of H2O2 in the reaction system will cause oxidative decomposition of ferrous heme, resulting in the enzyme inactivation. To overcome this, regulating the concentration of H2O2 in the reaction is particularly important for the efficient use of peroxygenase. This paper outlines a variety of methods used for in situ generation of H2O2 to drive peroxygenases reported in recent years. The pros and cons of biocatalysis, photocatalysis and electrocatalysis used for high atom-efficient H2O2 generation are comprehensively analyzed. This review paper is expected to provide a reference for promoting the application of peroxygenases in organic synthesis.

Key words: peroxygenase, H2O2 in situ generation, C—H bond oxyfunctionalization, biocatalysis, organic synthesis