### 水-乙醇混合溶剂中过氧化氢酶活性与构象的研究

1. 浙江大学化学系.杭州(310027)
• 发布日期:2000-06-15

### Activity and conformation of catalase in water-ethanol mixtures

Yi Pinggui;Yu Qingsen;Hu Xingen;Shang Zhicai;Mei Minghui;Lin Ruisen

1. Zhejiang Univ, Dept Chem.Hangzhou(310027)
• Published:2000-06-15

The activity and conformation changes of catalase in water-ethanol mixtures were measured at 298.15K by means of microcalorimetry and fluorescence spectroscopy. The results showed that the Michaelis constant K~m of catalase catalyzed reaction as well as the relative fluorescence intensity of catalase increased the rate constant k~2 and the activity of catalase decreased with increasing the ethanol concentration. The conformation of catalase was changed with losing activity at the various concentration of ethanol. The lose of activity of catalase in water-ethanol mixtures was caused by the inhibition of ethanol and the conformational change of catalase.