有机化学 ›› 2019, Vol. 39 ›› Issue (4): 916-928.DOI: 10.6023/cjoc201807055 上一篇    下一篇

所属专题: 荧光探针-生物传感合辑 有机超分子化学合辑

综述与进展

巯基类荧光探针的研究进展

闫沛沛a, 王婷b, 张丹b, 马晓雪a   

  1. a 山东医药技师学院 泰安 271000;
    b 贵州大学化学与化工学院 贵阳 550000
  • 收稿日期:2018-07-31 修回日期:2018-10-11 出版日期:2019-04-25 发布日期:2018-12-28
  • 通讯作者: 闫沛沛 E-mail:1458851831@qq.com

Progress in Fluorescence Sensors for Detection of Thiols

Yan Peipeia, Wang Tingb, Zhang Danb, Ma Xiaoxuea   

  1. a Shandong Medical Technician College, Taian 271000;
    b School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550000
  • Received:2018-07-31 Revised:2018-10-11 Online:2019-04-25 Published:2018-12-28
  • Contact: 10.6023/cjoc201807055 E-mail:1458851831@qq.com

生物硫醇类物质,如半胱氨酸、高半胱氨酸、谷胱甘肽等,在生命体中有至关重要的生理和病理作用.因此,近几年对生物硫醇的研究成了一大热点.目前巯基类荧光探针的设计主要是基于巯基的强亲核性.根据荧光探针与巯基反应的不同的机理,综述了近几年关于检测硫醇的荧光探针的合成报道,从迈克尔加成、醛基的环化、裂解、取代和其他几个方面对探针的检测限、响应时间、当量比、选择性、稳定性等进行了综述.

关键词: 巯基, 荧光探针, 生物硫醇, 研究进展

Sulfhydryl compounds, such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and so on, play an important role in the normal human physiological processes. Molecular fluorescent probes have attracted much attention of scholar in the detection of sulfhydryl compounds, since it has the advantage of good selectivity, high sensitivity, good biocompatibility and real-time in situ monitoring. At present, the design of thiol-based fluorescent probes is mainly based on the strong nucleophilicity of sulfhydryl groups. In this paper, the synthesis of fluorescent probes for detecting sulfhydryl compounds, which was reported from 2013 to 2018, is reviewed based on the different mechanism of the reaction between fluorescence probe and sulfhydryl, including Michael addition, cyclization of aldehyde groups, sulfonamides or sulfonates of the lysis, natural chemical linkage and so on. The detection limit of the probe, response time, equivalence ratio were elaborated.

Key words: thiols, fluorescent probe, biological thiols, research progress