有机化学 ›› 2020, Vol. 40 ›› Issue (1): 181-185.DOI: 10.6023/cjoc201907039 上一篇    下一篇

研究简报

一种基于协同效应的肼荧光探针及其在细胞成像中的应用

胥稳智, 李雪, 韩孟楠, 周婷婷, 杨瑜涛, 李玮   

  1. 河北大学化学与环境科学学院 药物化学与分子诊断教育部重点实验室 河北省化学生物学重点实验室 河北保定 071002
  • 收稿日期:2019-07-26 修回日期:2019-08-28 出版日期:2020-01-25 发布日期:2019-09-25
  • 通讯作者: 胥稳智, 李玮 E-mail:wzxu2008@sinano.ac.cn;liweihebeilab@163.com
  • 基金资助:
    国家自然科学基金(Nos.21702043,21807021)资助项目.

A Fluorescent Probe for Hydrazine Based on Synergistic Effect and Its Utilization in Cell Imaging

Xu Wenzhi, Li Xue, Han Mengnan, Zhou Tingting, Yang Yutao, Li Wei   

  1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, Hebei 071002
  • Received:2019-07-26 Revised:2019-08-28 Online:2020-01-25 Published:2019-09-25
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Nos. 21702043, 21807021).

开发了一种基于协同效应的新型肼荧光探针.通过在发色团上引入两种不同的识别位点,双识别位点的协同效应使得该探针对肼具有良好的专一性和灵敏度,检测限可达0.05~10.0μmol·L-1.此外,该探针还可用于细胞(Bt-474)中肼的检测.

关键词: 水合肼, 协同效应, 细胞成像, 荧光探针

Hydrazine is an important raw material and catalyst in chemical industry. However, hydrazine is very harmful to human organs. A new type of probe was developed based on the synergistic effect. Two recognition sites were introduced into the probe to improve performance to hydrazine, which has good specificity to hydrazine. The limit of detection (LOD) for N2H4 was 0.05~10.0 μmol·L-1. Moreover, the probe could detect N2H4 in BT-474 cells.

Key words: hydrazine, synergistic effect, cell imaging, fluorescent probe