A New Fluorescent Probe for Fe3+ and Its Application to Bioimaging

doi:10.6023/cjoc201709017

Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (3): 636-641.DOI: 10.6023/cjoc201709017 Previous Articles Next Articles

Special Issue: 荧光探针-生物传感合辑；有机超分子化学合辑

Articles

新型Fe³⁺荧光探针及其生物成像研究

杨美盼^a,b, 苏娜^c, 李玉香^a,b, 王莉^a,b, 马利锋^a,b, 张媛^a,b, 李靖^a,b, 杨秉勤^c, 康龙丽^a,b

a 高原相关疾病分子遗传机制与干预研究重点实验室西藏民族大学咸阳 712082;
b 高原环境与疾病相关基因研究重点实验室西藏民族大学咸阳 712082;
c 合成与天然功能分子化学教育部重点实验室西北大学西安 710127

收稿日期:2017-09-11 修回日期:2017-11-24 发布日期:2017-12-01
通讯作者: 杨美盼,E-mail:ymp1111@126.com E-mail:ymp1111@126.com
基金资助:
西藏自治区自然科学基金（（No.XZ2017ZGR-61）和西藏民族大学"青年学人培育计划"（No.17MDQP03）资助项目.

A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging

Yang Meipan^a,b, Su Na^c, Li Yuxiang^a,b, Wang Li^a,b, Ma Lifeng^a,b, Zhang Yuan^a,b, Li Jing^a,b, Yang Bingqin^c, Kang Longli^a,b

a Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease, Xizang Minzu University, Xianyang 712082;
b Key Laboratory of High Altitude Environment and Gene Related to Disease, Xizang Minzu University, Xianyang 712082;
c Key Laboratory for Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi'an 710127

Received:2017-09-11 Revised:2017-11-24 Published:2017-12-01
Contact: 10.6023/cjoc201709017 E-mail:ymp1111@126.com
Supported by:
Project supported by the Natural Science Foundation of Xizang (Tibet) Autonomous Region (No. XZ2017ZGR-61) and "The Training Plan for the Youth" Support Program of Xizang Minzu University (No. 17MDQP03).

1. A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging.PDF(450KB)

Abstract

A turn-on probe, which exhibited highly selective and sensitive signaling behaviors toward Fe³⁺ over other common metal ions, has been synthesized and confirmed by ¹H NMR, ¹³C NMR, IR and MS. The addition of Fe³⁺ to the solution of probe induced a remarkable UV-Vis and fluorescence enhancement along with obvious color change detected by the naked eye. The reaction mechanism was also investigated and proposed as that the probe complexed Fe³⁺ via a 1:1 binding mode, and the subsequent complexation caused the ring-opening of the rhodamine spirolactam and thereby the changes of absorbance and fluorescence. These remarkable properties may allow Fe³⁺ to be detected directly in the presence of the other examined competing metal ions. Then, cells experiments showed that the probe could permeate through cell membranes and react to Fe³⁺ within living cells. Additionally, the probe was further used for detecting Fe³⁺ ions in living mouse which exhibited excellent fluorescence imaging.

Key words: fluorescent probe, Fe³⁺, spectral properties, mechanism, cells and mouse

Cite this article

Yang Meipan, Su Na, Li Yuxiang, Wang Li, Ma Lifeng, Zhang Yuan, Li Jing, Yang Bingqin, Kang Longli. A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging[J]. Chin. J. Org. Chem., 2018, 38(3): 636-641.

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新型Fe³⁺荧光探针及其生物成像研究

A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging

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新型Fe3+荧光探针及其生物成像研究