Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (3): 793-799.DOI: 10.6023/cjoc201807036 Previous Articles     Next Articles

Articles

系列红色染料的合成、光学性质及在蛋白标记中的应用

李慧芳, 冷鑫, 闫立润, 杨秉勤, 白银娟   

  1. 西北大学化学与材料科学学院 合成与天然功能分子化学教育部重点实验室 西安 710127
  • 收稿日期:2018-07-22 修回日期:2018-09-11 出版日期:2019-03-25 发布日期:2018-10-26
  • 通讯作者: 杨秉勤, 白银娟 E-mail:yangbq@nwu.edu.cn;baiyinjuan@nwu.edu.cn
  • 基金资助:

    国家自然科学基金(No.21172178)资助项目.

A Series of Red Dyes: Synthesis, Optical Properties and Application in Protein Labeling

Li Huifang, Leng Xin, Yan Lirun, Yang Bingqin, Bai Yinjuan   

  1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127
  • Received:2018-07-22 Revised:2018-09-11 Online:2019-03-25 Published:2018-10-26
  • Contact: 10.6023/cjoc201807036 E-mail:yangbq@nwu.edu.cn;baiyinjuan@nwu.edu.cn
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21172178).

A series of red fluorescent dyes were synthesized using 3-N,N-diethylaminophenol. Their structures were characterized by 1H NMR, 13C NMR, IR and HRMS. The optical properties of these dyes were detected. Bovine serum albumin was labeled by new dyes. The fluorescence signal values of these dyes and cyanine-3 (Cy3) in lectin microarrays were compared. The results show that the maximum emission wavelengths of these dyes are above 600 nm and the Stokes shifts are about 70 nm. The dye/protein (D/P) labeling efficiencies of these dyes are as high as 1.5~1.8. The fluorescence signal of new dyes is equal to Cy3 in lectin microarray. Hence, these dyes can also be used as fluorescent labels for detection of sugar chain changes in lectin microarrays.

Key words: fluorescent dye, synthesis, spectral property, protein label, lectins microarray