Chin. J. Org. Chem. ›› 2019, Vol. 39 ›› Issue (3): 591-616.DOI: 10.6023/cjoc201810013 Previous Articles     Next Articles

Special Issue: 荧光探针-生物传感合辑



矫春鹏a,b,c,d, 刘媛媛a,b,c,d, 路文娟a,b,c,d, 张平平a,b,c,d, 王延风a,b,c,d   

  1. a 济南大学山东省医学科学院医学与生命科学学院 济南 250062;
    b 山东省医学科学院药物研究所 济南 250062;
    c 国家卫生部生物技术药物重点实验室 济南 250062;
    d 山东省罕少见病重点实验室 济南 250062
  • 收稿日期:2018-10-12 修回日期:2018-11-02 发布日期:2018-11-30
  • 通讯作者: 王延风
  • 基金资助:


Molecular Fluorescence Probe for Detecting Reactive Nitrogen/Reactive Oxygen

Jiao Chunpenga,b,c,d, Liu Yuanyuana,b,c,d, Lu Wenjuana,b,c,d, Zhang Pingpinga,b,c,d, Wang Yanfenga,b,c,d   

  1. a School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062;
    b Institute of Materia Medica Shandong Academy of Medical Sciences, Jinan 250062;
    c Key Laboratory for Biotech-Drugs Ministry of Health, Jinan 250062;
    d Key Laboratory of Rare and Uncommon Diseases of Shandong Province, Jinan 250062
  • Received:2018-10-12 Revised:2018-11-02 Published:2018-11-30
  • Contact: 10.6023/cjoc201810013
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21305079).

Reactive nitrogen and reactive oxygen are chemical substances with strong biological activity. In human cells, peroxides can be generated due to enzymatic or non-enzymatic processes. Abnormal levels of peroxide can cause oxidative damage and aging and various diseases such as cardiovascular disease, neurological diseases, Alzheimer's disease, Parkinson's disease and even cancer. In order to effectively cure these diseases, health workers must find the source of the problem. Currently, there is no better way to detect reactive oxygen species and reactive nitrogen. Fluorescence spectrometry in recent years becomes the preferred method for the majority of researchers for detecting active oxygen and reactive nitrogen. Therefore, the development of selective recognition and high sensitivity molecular fluorescent probes to achieve effective detection of reactive nitrogen and reactive oxygen species is of great significance. On one hand, molecular fluorescent probe detecting and imaging technology has excellent characteristics such as high sensitivity, strong selectivity, small damage and good cell compatibility. On the other hand, fluorescent probes play an important role in the pathophysiological process of reactive nitrogen and reactive oxygen species. Therefore, the fluorescent probe method is widely used in the fields of biology and medicine. However, due to the inherent specificity of reactive nitrogen and reactive oxygen species, it has become an urgent problem for researchers, such as high reactivity, short cycle, etc. In order to overcome the shortcomings of fluorescent probe analysis, researchers are constantly striving to find better active fluorescent probes for the detection of reactive nitrogen and reactive oxygen species. Recent evolutions in the development of molecular fluorescent probes for the detection of active nitrogen and reactive oxygen species and cell imaging work are reviewed. Finally, a new type of molecular fluorescent probe is proposed to be used for the challenge of active nitrogen and active oxygen detection, and the future development direction and prospect.

Key words: reactive nitrogen species, reactive oxygen species, fluorescence probe, fluorescence imaging