Chinese Journal of Organic Chemistry ›› 2022, Vol. 42 ›› Issue (5): 1375-1386.DOI: 10.6023/cjoc202112022 Previous Articles     Next Articles



李娜, 谭啸峰*(), 杨晴来*()   

  1. 南华大学衡阳医学院 基础医学院 肿瘤研究所 湖南省肿瘤细胞与分子病理学重点实验室分子影像探针实验室 湖南衡阳 421001
  • 收稿日期:2021-12-17 修回日期:2022-01-22 发布日期:2022-02-10
  • 通讯作者: 谭啸峰, 杨晴来
  • 基金资助:
    国家自然科学基金(81801749); 湖南省重点研发计划(2022SK2053); 湖南省科技创新计划“湖湘青年英才”(2021RC3106)

Recent Progress on Strategies and Applications of Imaging for Intestinal Microflora

Na Li, Xiaofeng Tan(), Qinglai Yang()   

  1. Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, School of Preclinical Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan 421001
  • Received:2021-12-17 Revised:2022-01-22 Published:2022-02-10
  • Contact: Xiaofeng Tan, Qinglai Yang
  • Supported by:
    National Natural Science Foundation of China(81801749); Key Research and Development Program of Hunan Province(2022SK2053); Science and Technology Innovation Program of Hunan Province “Huxiang Young Talents Plan”(2021RC3106)

The microflora in the mammalian gut plays an essential role in maintaining the physiological states and pathological changes of the host, and it is of significance for host health to detect the microflora in the gut. However, the traditional staining methods for bacteria have some drawbacks, such as poor specificity, incompatibility with living cells, and susceptibility, thereby limiting further study on the morphology and function of intestinal microflora. The fluorescence imaging technique could accurately reveal the location of symbiotic, pathogenic, and opportunistic bacteria in the intestine. The fluorescence imaging technique could reflect some information, including bacterial activity, metabolic exchange, and immune interactions between the host and the microbe due to its advantages of non-invasive, less tissue damage, higher specificity, and sensitivity, thereby being widely utilized in bacterial detection. More importantly, the fluorescent-labeled probes are the critical factor in the fluorescence imaging of intestinal microflora, promoting the development of fluorescence imaging technology continuously. This review summarizes the labeling strategies designed for different intestinal microflora, including fluorescent probes and isotope probes, and disscussed the metabolic labeling, the methods of non-metabolic labeling and the specific labeling of metabolites, finally providing a prospect for the development of the fluorescent-labeled probes for intestinal microflora.

Key words: intestinal microflora imaging, fluorescent probes, bioorthogonal reaction, peptidoglycan labeling, D-amino acid metabolic labeling