巨大芽孢杆菌D01吸附金(Au3+)的谱学表征

化学学报 ›› 2004, Vol. 62 ›› Issue (18): 1829-1834. 上一篇下一篇

研究论文

巨大芽孢杆菌D01吸附金(Au³⁺)的谱学表征

林种玉¹, 吴剑鸣¹, 傅博强¹, 薛茹¹, 周剑章¹, 郑泉兴¹, 刘月英², 傅锦坤¹

1. 厦门大学化学系, 固体表面物理化学国家重点实验室, 厦门, 361005;
2. 厦门大学化学系, 生物系, 厦门, 361005

投稿日期:2004-03-25 修回日期:2004-06-24 发布日期:2014-02-17
通讯作者: 林种玉,E-mail:stzylin@xnu.edu.cn;Fax:0592-2183047. E-mail:stzylin@xnu.edu.cn

Spectroscopic Characterization on Interaction of Gold (Au³⁺) Biosorption by Bacillus megaterium DO1

LIN Zhong-Yu¹, WU Jian-Ming¹, FU Bo-Qiang¹, XUE Rua¹, ZHOU Jian-Zhang¹, ZHENG Quan-Xing¹, LIU Yue-Ying², FU Jin-Kun¹

1. Department of Chemistry, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005;
2. Department of Biology, Xiamen University, Xiamen 361005

Received:2004-03-25 Revised:2004-06-24 Published:2014-02-17

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摘要/Abstract

对休眠的巨大芽孢杆菌(Bacillus megatheriun)D01菌体吸附Au³⁺的作用过程进行了谱学表征.运用AAS考察了pH、时间和温度对D01菌体吸附Au³⁺过程的化学动力学和热力学相关参数的影响.D01菌粉中硫元素含量的EDX分析说明该菌体中对Au³⁺具有还原作用的L-半胱氨酸和蛋氨酸的含量极少;D01菌体水解后葡萄糖含量的UV-vis测定说明该菌体水解产物中含有一定量的还原糖.空白的和吸附Au³⁺的D01菌体的FTIR检测表明该菌体细胞壁肽聚糖层糖类化合物的羟基和肽链侧链氨基酸残基离子化羧基为吸附Au³⁺的活性基团;肽聚糖层部分多糖的水解产物低聚糖、二糖及单糖等还原糖的半缩醛羟基游离态醛基为电子供体,将Au³⁺原位还原成Au0.葡萄糖和Au³⁺相互作用的XRD和FTIR表征证明Au³⁺是在还原糖的醛基上直接被还原成Au0.

关键词: 生物吸附, 巨大芽孢杆菌, 金, 生物还原, FTR

Biosorptive interaction of gold with resting cell of Bacillus megatherium D01 biomass has been characterized by spectroscopic techniques. The effects of pH, time and temperature on the correlation parameters of chemical kinetics and thermodynamics of the binding reaction has been investigated through the determination of the gold ion binding to the biomass using atomic absorption spectrophotometry (AAS). The analysis for sulfur content in dry powder of the D01 biomass by energy dispersive X-ray (EDX) shows that cysteine and methionine being capable of reducing Au³⁺ to Au⁰ is very small, whereas the glucose content in hydrolysates of the biomass analyzed by ultraviolet-visible spectrophotometry (UV-vis) indicates that the amount of the reducing sugars in the biomass is much larger than 3.33%. The Fourier transform infrared (FTIR) spectroscopy on blank and gold-loaded biomass demonstrates that the active groups such as the hydroxyl group of saccharides and the ionized carboxyl group of amino acid residues on the cell wall seem to be the sites for Au³⁺ binding, and the free aldehyde group of the hemiacetalic hydroxyl group from reducing sugars, i.e. the hydrolysates of the polysaccharides, serving as the electron donor, in situ reduces the Au³⁺ to Au⁰. X-ray powder diffractometry (XRD) and FTIR spectroscopic characterizations of the interaction of Au³⁺ with glucose confirm that the reduction of Au³⁺ to Au⁰can directly occur in the aldehyde group of the reducing sugars.

Key words: biosorption, Bacillus megatherium, gold, bioreduction, FTIR

引用此文

林种玉, 吴剑鸣, 傅博强, 薛茹, 周剑章, 郑泉兴, 刘月英, 傅锦坤. 巨大芽孢杆菌D01吸附金(Au³⁺)的谱学表征[J]. 化学学报, 2004, 62(18): 1829-1834.

LIN Zhong-Yu, WU Jian-Ming, FU Bo-Qiang, XUE Rua, ZHOU Jian-Zhang, ZHENG Quan-Xing, LIU Yue-Ying, FU Jin-Kun. Spectroscopic Characterization on Interaction of Gold (Au³⁺) Biosorption by Bacillus megaterium DO1[J]. Acta Chimica Sinica, 2004, 62(18): 1829-1834.

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巨大芽孢杆菌D01吸附金(Au³⁺)的谱学表征

Spectroscopic Characterization on Interaction of Gold (Au³⁺) Biosorption by Bacillus megaterium DO1

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