烷基聚葡糖苷微乳液中脂肪酶催化的酯合成反应动力学

化学学报 ›› 2004, Vol. 62 ›› Issue (19): 1894-1900. 上一篇下一篇

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烷基聚葡糖苷微乳液中脂肪酶催化的酯合成反应动力学

柴金岭^1,2, 高延红¹, 王金山¹, 李干佐², 张高勇², 徐清³

1. 山东师范大学化学系, 济南, 250014;
2. 山东大学胶体与界面化学教育部重点实验室, 济南, 250100;
3. 诺维信(中国)有限公司, 北京, 100085

投稿日期:2003-12-12 修回日期:2004-05-07 发布日期:2014-02-17
通讯作者: 柴金岭,E-mail:jlchai99@sina.com E-mail:jlchai99@sina.com
基金资助:
山东省自然科学基金(No.Y2003B01)资助项目.

Kinetics of Esterication Reaction Catalyzed by Lipase in W/O Microemulsions of Alkyl Polyglucoside

CHAI Jin-Ling^1,2, GAO Yan-Hong¹, WANG Jin-Shan¹, LI Gan-Zuo², ZHANG Gao-Yong², XU Qing³

1. Department of Chemistry, Shandong Normal University, Jinan 250014;
2. Key Laboratory for Colloid and Interface Chemistry of State Education Ministry, Shandong University, Jinan 250100;
2. Novozym Company of Beijing, Beijing 100085

Received:2003-12-12 Revised:2004-05-07 Published:2014-02-17

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摘要/Abstract

在烷基聚葡糖苷C₁₀G_1.54/正丁醇/环己烷/磷酸盐缓冲液体系形成的油包水微乳液中,研究了在较高底物浓度下,脂肪酶催化正己酸和正丁醇的酯合成反应动力学及其影响因素.结果表明,在正己酸的初始浓度C0HA较小时,反应初速率V₀随正己酸浓度增大而增大,但随正丁醇表观浓度增大而减小;在C_HA⁰较大时,反应初速率V₀随正丁醇表观浓度增大而增大,但随正己酸浓度增大而减小.在正己酸和正丁醇摩尔浓度之比为3.0左右反应速率最大.这些实验结果与传统的米氏反应不符,为此,从界面膜的构成,尤其是两种底物在界面膜上的分布情况入手进行了探讨.考察了C₁₀G_1.54浓度、正丁醇浓度和两者总浓度等对反应初速率V₀的影响.

关键词: 烷基聚葡糖苷, 油包水微乳液, 脂肪酶催化, 动力学

The kinetics and its influencing factors of esterication reaction of hexanoic acid with n-butanol, catalyzed by lipase, were studied in water-in-oil microemulsions formed by alkyl polyglucoside C₁₀G_1.54/n-butanol/cyclohexane/phosphate buffer solution system. The result shows that the initial rate V₀ of the esterification reaction increases as hexanoic acid concentration increases or as n-butanol concentration decreases, when the initial concentration of hexanoic acid is small; and the initial rate V₀ decreases as hexanoic acid concentration increases or as n-butanol concentration decreases, when the initial concentration of hexanoic acid is large. When the molar concentration ratio of n-butanol to hexanoic acid is about 3.0, the initial rate V₀ gets the maximum value, which is explained with the fish-like phase diagram. In addition, the effects of the lipase concentration, the pH values of water buffer solutions, and the kinds of hydrocarbon molecules and alcohols on the kinetics were also investigated.

Key words: alkyl polyglucoside, W/O microemulsion, lipase catalysis, kinetics

引用此文

柴金岭, 高延红, 王金山, 李干佐, 张高勇, 徐清. 烷基聚葡糖苷微乳液中脂肪酶催化的酯合成反应动力学[J]. 化学学报, 2004, 62(19): 1894-1900.

CHAI Jin-Ling, GAO Yan-Hong, WANG Jin-Shan, LI Gan-Zuo, ZHANG Gao-Yong, XU Qing. Kinetics of Esterication Reaction Catalyzed by Lipase in W/O Microemulsions of Alkyl Polyglucoside[J]. Acta Chimica Sinica, 2004, 62(19): 1894-1900.

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烷基聚葡糖苷微乳液中脂肪酶催化的酯合成反应动力学

Kinetics of Esterication Reaction Catalyzed by Lipase in W/O Microemulsions of Alkyl Polyglucoside

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