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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (23): 2153-2157. 上一篇    下一篇

研究论文

全反式维甲酸合铜(II)配合物对DNA的切割和键合作用

杨培菊1,2, 孙坤3, 苏雪3, 王流芳4, 宋玉民1   

  1. 1. 西北师范大学化学化工学院, 兰州, 730070;
    2.中国科学院兰州化学物理研究所, 羰基合成与选择氧化国家重点实验室, 兰州, 730000;
    3. 西北师范大学生命科学学院, 兰州, 730070;
    4. 兰州大学功能有机分子化学国家重点实验室, 兰州, 730000
  • 投稿日期:2004-12-01 修回日期:2005-06-07 发布日期:2014-02-14
  • 通讯作者: songym@nwnu.edu.cn
  • 作者简介:宋玉民,E-mail:songym@nwnu.edu.cn
  • 基金资助:

    国家自然科学基金(No.30270091);甘肃省自然科学基金(No.3zs051-Az5-021);甘肃省高分子材料重点实验室西北师范大学A级重点学科资助项目.

Study on the Interactions and Cleavage of Cu(RA)2·3H2O with DNA

YANG Pei-Jua1,2, SUN Kun3, SU Xue3, WANG Liu-Fang4, SONG Yu-Min1   

  1. 1. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070;
    2. State Key Laboratory of Carbonyl Synthesis and Oxidation, Institute of Chemistry and Physics, Chinese Academy of Sciences, Lanzhou 730000;
    3. College of Life Science, Northwest Normal University, Lanzhou 730070;
    4. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000
  • Received:2004-12-01 Revised:2005-06-07 Published:2014-02-14

PDF

679

被引次数

47

以荧光法、粘度法、凝胶电泳和电化学方法研究了全反式维甲酸合铜(Ⅱ)配合物与DNA的作用.结果表明,该配合物能在生理条件下有效切割质粒DNA,加入H2O2后发现该配合物的切割活性增强,说明该配合物对DNA的切割机理可能有两种:氧化和水解.同时可使DNA的粘度增加,使EB-DNA体系的荧光强度降低.DNA的存在能导致该配合物氧化还原峰电流降低.据此推断,该配合物主要以嵌入方式与DNA作用.

关键词: 全反式维甲酸合铜(II), 小牛胸腺DNA, 切割, 电化学

The interactions of the Cu(II) complex of all-trans retinoic acid [Cu(RA)2·3H2O] with DNA have been studied using agarose gel electrophoresis, fluorescence spectroscopy, viscosity measurement and electrochemical methods. The results indicate that the Cu(RA)2·3H2O can effectively promote the cleavage of plasmid DNA at physiological pH and temperature, and also increase the viscosity of DNA. The emission intensity was quenched as Cu(RA)2·3H2O was added to the EB-DNA system. The results above suggest that the complex should bind DNA mainly by intercalation, to cause a decrease of anodic peak current in their cyclic voltammograms and exhibit enhanced DNA binding.

Key words: Cu(RA)2·3H2O, calf thymus DNA, cleavage, electrochemistry