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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (5): 790-794. 上一篇    下一篇

研究论文

基于巯基自组装单层膜的植物生长激素吲哚乙酸电化学免疫传感器的研究

李春香;李劲;萧浪涛;沈国励;俞汝勤   

  1. 湖南大学化学化工学院;湖南农业大学
  • 发布日期:2003-05-15

Amperometric Immunosensor Based on Mercapto Carboxylic Acid Self- assembled Monolayer for Phytohormone Indoleacetic Acid Assay

Li Chunxiang;Li Jin;Xiao Langtao;Shen Guoli;Yu Ruqin   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University;Key Laboratory of Phytohormones, Hunan Agricultural University
  • Published:2003-05-15

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658

被引次数

84 | 10

提出了一种酶标识抗原与待测样品的竞争免疫反应定量测定IAA的方法．该方 法是基于金基底上形成了均一、稳定、有序的硫基自组装单层膜，能以共价方式固 定抗体．利用循环伏安法探讨了底物在不同条件下的反应特征，并研究了最佳实验 条件．在浓度5．68×10^-7～2.83×10^-5mol/L范围内、-200mV电位下，响应电流 与lg[IAA]呈线性关系，其回归方程；i(nA)=-20.8333×lg(10^6[IAA])+68.9167， 相关系数0.9915。对样品IAA的含量进行测定，结果令人满意．

关键词: 吲哚乙酸, 循环伏安法, 植物生长调节剂, 传感器, 电化学

A competitive binding assay used to detennine hapten IAA with the aid of HRP-IAA conjugate has been developed. It is based on the ordered, stable mercapto self-assembled monolayer formed on gold surface, which immobilized IgG with covalent bond. The features of the substrate reaction under different conditions were investigated by cyclic voltammetry and the assay conditions were optimized. Under applied potential of - 200 mV, the response current is linear with lg [IAA], over a concentration range of 5.68 X 10-7 ~ 2.83 X 10-5 mol/L with a correlation coefficient of 0.9915. The sensor was applied for the determination of IAA sample with satisfactory results.

Key words: INDOLEACETIC ACID, CYCLOVOLTAMGRAPH, PLANT GROWTH REGULATORS, SENSORS, ELECTROCHEMISTRY

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