关键词: 萘酚P, 多足胺类化合物, 光物理, 探针, ctDNA, 荧光猝灭, 脱氧核糖核酸, 相互作用

A series of multi-pedal amino-compound with naphthol groups have been synthesized in this work. The photo-physical behaviors of these compounds in different solvents and different pH conditions have also been studied in details. The properties of environment in which the probe molecule located were estimated properly by the ratio of emission intensity of naphthol and phenol anion peak wave-length in the fluorescence spectra of these compounds. The problem of fluorescence quenching of the studied compounds by ctDNA was also been investigated. The results indicate that the compound with tripedal has a stronger interaction with ctDNA than that of other compounds for which one or two pedals were contained. The interalation of naphthol group into double helix structure of ctDNA resulted in fluorescence quenching of naphthol group due to the interaction of this group and the base pair of DNA. It is due to the fact that the dual fluorescence emission of excited naphthol group had different quenching constants by ctDNA. The mechanism of this interesting phenomenon was disucssed preliminarily.

Key words: NAPHTHALENOL P, PHOTOPHYSICS, PROBES, DEOXYRIBONUCLEIC ACID, INTERACTIONS