化学学报 ›› 2009, Vol. 67 ›› Issue (24): 2862-2866. 上一篇    下一篇

研究论文

超高压微射流均质中花生球蛋白的机械力化学效应研究

张雪春1,涂宗财1,郑为完2,黎鹏2,陈钢2,王辉2,豆玉新2   

  1. 1. 南昌大学
  • 投稿日期:2009-05-05 修回日期:2009-07-23 发布日期:2010-02-04
  • 通讯作者: 涂宗财 E-mail:tuzc_mail@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(20666004)资助项目;教育部长江学者和创新团队发展计划项目(IRT0540)

Mechano-chemical Effect on Arachin by High
Pressure Microfluidization

ZHANG Xun-chun,zongcai Tu,Wei wan Zheng, , , ,   

  • Received:2009-05-05 Revised:2009-07-23 Published:2010-02-04
  • Contact: zongcai Tu E-mail:tuzc_mail@yahoo.com.cn

以花生球蛋白为原料,研究超高压微射流均质对花生球蛋白作用的机械力化学效应,通过电镜扫描(SEM)、SDS-PAGE、荧光光谱(FLU)和差热式扫描(DSC)对超高压微射流均质后花生球蛋白的微观形貌、亚基组成、表面疏水性和热稳定性进行分析。结果表明:在超高压微射流的机械力作用下,花生球蛋白的亚基和分子量几乎不受影响;花生球蛋白的颗粒和热稳定性随着处理压力的增大而减小,分别在80 MPa和120 MPa时达到最小;表面疏水性随着处理压力的增大而增强,在160 MPa时达到最大。超高压微射流均质过程的机械力化学效应可使花生球蛋白的微观形貌和三维结构改变,热稳定性降低。

关键词: 机械力化学效应, 超高压微射流均质, 花生球蛋白, 微观结构, 热稳定性

Mechano-chemical effects on arachin in the process of high pressure microfluidization (HPM)
were studied. The micro-morphology, subunits, surface hydrophobicity and the thermal stability of homogenized
arachin were analyzed by scanning electron microscopy (SEM), SDS-PAGE, fluorescence spectra
(FLU) and differential scanning calorimetry (DSC). The results showed that the subunits and the molecular
weight of arachin were not affected by HPM. The particle size and the thermal stability of arachin decreased
with the pressure increasing, and the minima were observed at 80 and 120 MPa, respectively. The surface
hydrophobicity of arachin increased with the pressure increasing, and the maximum was observed at 160
MPa. The mechano-chemical effects of HPM can change the micro-morphology and three dimensional
structure of arachin, and decrease the thermal stability of arachin.

Key words: mechano-chemical effect, high pressure microfluidization, arachin, microstructure, thermal stability