化学学报 ›› 2012, Vol. 70 ›› Issue (20): 2127-2132.DOI: 10.6023/A12080499 上一篇    下一篇

研究通讯

表面引发的DNA杂交链式反应的石英晶体微天平研究

王萍, 葛志磊, 裴昊, 王丽华, 樊春海   

  1. 中国科学院上海应用物理研究所物理生物学实验室 上海 201800
  • 投稿日期:2012-08-03 发布日期:2012-09-05
  • 通讯作者: 樊春海 E-mail:fchh@sinap.ac.cn
  • 基金资助:

    项目受国家自然科学基金(No. 21075128)和上海市科委(No. 10QA1408200)资助.

Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction

Wang Ping, Ge Zhilei, Pei Hao, Wang Lihua, Fan Chunhai   

  1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800
  • Received:2012-08-03 Published:2012-09-05
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21075128) and Shanghai Municipal Commission for Science and Technology (No. 10QA1408200).

通过石英晶体振荡技术研究了杂交链式反应这种核酸扩增的方法. 石英晶体微天平可以表征在晶体和溶液的界面上的DNA层, 并获得粘性穿透深度这一重要参数. 根据石英晶体表面吸附质量和振荡频率之间的关系, 我们测量了表面引发的杂交链式反应的动力学过程, 并获得界面上的粘度、剪切模量等参数. 这一工作为研究固液界面上核酸反应过程, 特别是杂交链式反应的机制提供了新的途径.

关键词: 杂交链式反应, 石英晶体微天平, DNA, 粘度, 界面, 穿透深度

The hybridization chain reaction (HCR), a type of nucleic acid amplification method, was studied by a quartz crystal resonator technique. We probed the properties of the boundary DNA layer with a thickness of the viscous penetration depth at the crystal-solution interface. According to the relationship between the change of resonant frequency and the mass adsorbed on the crystal surface, the kinetics of surface-initiated HCR was measured, as well as the viscosity and the shear modulus of H1/H2 solution with different concentrations near a solid substrate. This work provides a new method for studying nucleic acid reactions at solid surface and mechanistic insight for HCR-based amplification and biodetection.

Key words: hybridization chain reaction, quartz crystal microbalance, DNA, viscosity, interface, penetration depth