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Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (2): 137-141. Previous Articles     Next Articles

抗癌药长春新碱及其与微管蛋白相互作用的电化学研究

于泳1,2, 胡劲波1, 尚军1, 李启隆1   

  1. 1. 北京师范大学化学系, 北京, 100875;
    2. 中国科学院力学研究所微重力实验室, 北京, 100080
  • 投稿日期:2003-03-23 修回日期:2003-07-14 发布日期:2014-01-26
  • 通讯作者: 胡劲波,E-mail:hujingbo@bnu.edu.cn;Tel:(010)62209398;Fax:(010)62200567 E-mail:hujingbo@bnu.edu.cn
  • 基金资助:
    国家自然科学基金(No.20275007)资助项目.

Study on the Electrochemical Behaviors of Vincristine andthe Interaction of Vincristine with Tubulin

YU Yong1,2, HU Jing-Bo1, SHANG Jun1, LI Qi-Long1   

  1. 1. Department of Chemistry, Beijing Normal University, Beijing 100875;
    2. National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080
  • Received:2003-03-23 Revised:2003-07-14 Published:2014-01-26

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A sensitive reduction peak of vincristine (VCR) is obtained by adsorptive stripping voltammetry in 0.05 mol/L Tris and 0.15 mol/L NaCl buffer solution. The peak potential is -1.68 V (vs.Ag/AgCl). The peak current is directly proportional to the concentration of VCR (1.0×10-8 ～2 0×10-7 mol/L), with a detection limit of 7.0×10-9 mol/L. The electrochemical behavior of this system was studied by the methods of normal pulse polarography, linear sweep and cyclic voltammetry. The reduction process is irreversible with adsorptive characteristics. The adsorption of VCR obeys the Frumkin isotherm. The electrochemical behavior of the binding of VCR to tubulin was studied by cyclic voltammetry. The results showed that the reaction of tubulin dimer with VCR formed an electrochemically active complex, and the reduction process of the complex is irreversible.

Key words: vincristine(VCR), voltammetry, adsorption, tubulin