有机化学 ›› 2020, Vol. 40 ›› Issue (5): 1367-1371.DOI: 10.6023/cjoc201912012 上一篇    下一篇

研究简报

海洋真菌赤褐炭团菌FS521的次级代谢产物研究

张捷a,b, 陈玉婵a, 刘昭明a, 郭波红b, 高晓霞b, 刘洪新a, 章卫民a   

  1. a 广东省微生物研究所 广东省科学院 华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室 广东省微生物应用新技术公共实验室;
    b 广东药科大学药学院 广州 510006
  • 收稿日期:2019-12-09 修回日期:2020-01-05 发布日期:2020-01-15
  • 通讯作者: 刘洪新, 章卫民 E-mail:liuhx@gdim.cn;wmzhang@gdim.cn
  • 基金资助:
    国家自然科学基金(No.41906106,31272087)、广州市珠江科技新星(No.201806010080)、广东省自然科学基金研究团队(No.2016A030312014)和广东省科学院(No.2019GDASYL-0103007)资助项目.

Cytotoxic Secondary Metabolites from a Sea-Derived Fungal Strain of Hypoxylon rubiginosum FS521

Zhang Jiea,b, Chen Yuchana, Liu Zhaominga, Guo Bohongb, Gao Xiaoxiab, Liu Hongxina, Zhang Weimina   

  1. a State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070;
    b School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006
  • Received:2019-12-09 Revised:2020-01-05 Published:2020-01-15
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Nos. 41906106, 31272087), the Pearl River Science and Technology New Star Fund of Guangzhou Province (No. 201806010080), the Team Project of the Natural Science Foundation of Guangdong Province (No. 2016A030312014), and the Project of Science and Technology Development of Guangdong Academy of Sciences (No. 2019GDASYL-0103007).

从海洋真菌赤褐炭团菌(Hypoxylon rubiginosum)FS521中分离纯化得到17个聚酮类化合物,分别鉴定为4-乙酰氧基-8-甲氧基-3,4-二氢萘-1(2H)-酮(1)、4,8-二甲氧基-1-萘酚(2)、1'-羟基-4',8,8'-三甲氧基[2,2']联萘-1,4-二酮(3)、3,6-dimethyl-atromentin(4)、4-羟基-8-甲氧基-3,4-二氢萘-1(2H)-酮(5)、regiolone(6)、methylsclerone(7)、5-methylmellein(8)、3,5-二甲基-8-甲氧基-3,4-二羟基异香豆素(9)、9-甲氧基萘(10)、1,8-二甲氧基萘(11)、8-甲氧基-1-萘醇(12)、7-甲氧基-3-甲基异苯并呋喃(13)、3-甲基-4-羟基苯乙酸(14)、nodulisporipyrone A(15)、nodulisporipyrone B(16)、3,4-二甲氧基-乙酰苯乙醇(17).其中化合物1为新化合物,化合物23为新天然产物,新化合物的结构通过HRESIMS,1D NMR和2D NMR等光谱技术确定.此外,对分离所得的化合物14进行了体外细胞毒活性测试,结果表明化合物3对SF-268、MCF-7、HepG-2、A549有较强的细胞毒活性,其IC50值分别为1.85、3.21、2.53、5.09 mmol·L-1.

关键词: 海洋真菌, 赤褐炭团菌, 细胞毒活性, 聚酮

One new compound, named hypoxone A (1), together with two new natural products 4,8-dimethoxy-1-naphthol (2) and 1'-hydroxy-4',8,8'-trimethoxy[2,2']binaphthalenyl-1,4-dione (3), and fourteen known derivatives 4~17 were isolated from the broth extract of the marine fungus Hypoxylon rubiginosum FS521, which was isolated from a deep-sea sediment sample. Their structures were elucidated by interpretation of spectroscopic analysis and comparison with previously published data for the known ones. Moreover, compounds 1~4 were evaluated for their in vitro cytotoxic activities against SF-268, MCF-7, HepG-2, and A549 tumor cell lines, and compound 3 exhibited potent cytotoxic activity with IC50 values of 1.85, 3.21, 2.53, and 5.09 mmol·L-1, respectively.

Key words: marine fungus, Hypoxylon rubiginosum, cytotoxicity, polyketides