海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究

doi:10.6023/cjoc202507017

有机化学 ›› 2026, Vol. 46 ›› Issue (2): 684-688.DOI: 10.6023/cjoc202507017 上一篇下一篇

研究简报

海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究

王见见^a, 郑圣䶮^a, 张洁^a, 高文鹏^a, 孔凡栋^a, 李培海^b, 王聪^a^,^*(), 曹树更^c^,^*()

^a 广西民族大学化学化工学院林产化学与工程国家民委重点实验室广西林产化学与工程重点实验室林木生物质低碳高质利用广西高校工程研究中心中国广西省南宁市 530006
^b 齐鲁工业大学(山东省科学院)生物研究所山东省斑马鱼人类疾病模型与药物筛选工程技术研究中心中国山东省济南市 250103
^c 夏威夷大学希洛分校药学院药学系美国夏威夷州希洛市 96720

收稿日期:2025-07-14 修回日期:2025-08-23 发布日期:2025-10-14
通讯作者: 王聪, 曹树更
基金资助:
国家自然科学基金青年(82204276); 广西自然科学基金(2025GXNSFAA069557); 广西高等学校千名中青年骨干教师培育计划、广西民族大学研究生教育创新计划(gxmzu-chxs2024225)

Study on Secondary Metabolites of Marine-Derived Fungus Cladosporium sp. MDCW-211

Jianjian Wang^a, Shengyan Zheng^a, Jie Zhang^a, Wenpeng Gao^a, Fandong Kong^a, Peihai Li^b, Cong Wang^a^,^*(), Shugeng Cao^c^,^*()

^a Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi University Engineering Research Center for Low-carbon and High-quality Utilization of Forest Biomass, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning, Guangxi 530006, China
^b Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250103, China
^c Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawai'i at Hilo, Hilo, Hawai 96720, USA

Received:2025-07-14 Revised:2025-08-23 Published:2025-10-14
Contact: Cong Wang, Shugeng Cao
Supported by:
National Natural Science Foundation of China(82204276); Guangxi Natural Science Foundation(2025GXNSFAA069557); Guangxi Scholarship Fund of Guangxi Education Department, and the Innovation Project of Guangxi Minzu University Graduate Education(gxmzu-chxs2024225)

文章分享

1. 251015-1.pdf(1908KB)

摘要/Abstract

对北部湾涠洲岛来源的海洋真菌Cladosporium sp. MDCW-211产生的次级代谢产物及其生物活性进行研究. 利用制备型高效液相色谱(HPLC)等技术进行化合物的分离纯化, 运用高分辨率电喷雾质谱(HR-ESI-MS)、核磁共振波谱法(NMR)和X射线(X-ray)等方法对化合物1的结构进行鉴定, 采用斑马鱼模型评估化合物的促血管生成活性. 分离鉴定了1个新的二氢异香豆素类化合物1 (4'S-hydroxyasperentin)以及3个已知二氢异香豆素类化合物2~4, 其中化合物1为新的4'位为α-OH的二氢异香豆素类化合物. 活性结果表明, 化合物3在浓度为20和40 μmol/L时具有促血管生成活性, 同时报道了化合物3的促血管生成活性.

关键词: 海洋真菌, Cladosporium sp. MDCW-211, 二氢异香豆素类化合物, 促血管生成活性

A study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp. MDCW-211. Preparative high performance liquid chromatography (HPLC) and other techniques were used to isolate and purify the compounds, the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR) spectroscopy, X-ray and other methods, and the pro-angiogenic activity of the compounds was evaluated by zebrafish model. One new dihydroisocoumarin 1 (4'S-hydroxya- sperentin) and three known dihydroisocoumarins 2~4 were obtained and identified. Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40 μmol/L, which is reported here for the first time.

Key words: marine fungus, Cladosporium sp. MDCW-211, dihydroisocoumarins, pro-angiogenic activity

引用此文

王见见, 郑圣䶮, 张洁, 高文鹏, 孔凡栋, 李培海, 王聪, 曹树更. 海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究[J]. 有机化学, 2026, 46(2): 684-688.

Jianjian Wang, Shengyan Zheng, Jie Zhang, Wenpeng Gao, Fandong Kong, Peihai Li, Cong Wang, Shugeng Cao. Study on Secondary Metabolites of Marine-Derived Fungus Cladosporium sp. MDCW-211[J]. Chinese Journal of Organic Chemistry, 2026, 46(2): 684-688.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

图/表 4

Figure 1. Structures of compounds 1~4

Figure 2. Key 1H-1H COSY (bolds, blue) and HMBC (solid arrows, red) correlations of compound 1

Figure 3. X-ray crystallographic structure of compound 1

Figure 4. Influence of compounds on intersegmental vessels in zebrafish (A) Typical images of intersegmental vessels in zebrafish; (B) Quantitative analysis of intersegmental vessel length in zebrafish. Data represented as mean±SEM. Compared with the blank control group, ##: extremely significant difference (P<0.01); compared with the model group, **: extremely significant difference (P<0.01). Model, 0.2 µg/mL PTK787; FA, 40 μmol/L ferulic acid.

参考文献 16

[1]	Zhou C.; Li X.; Pan J.; Ge Y.; Wu X.; Wu B. J. Org. Chem. 2025, 90, 5125.
[2]	Wang C.; Guo L.; Hao J.; Wang L.; Zhu W. J. Nat. Prod. 2016, 79, 2977.
[3]	Kong F.; Wang Y.; Liu P.; Dong T.; Zhu W. J. Nat. Prod. 2014, 77, 132.
[4]	Saeed A. Eur. J. Med. Chem. 2016, 116, 290.
[5]	Liu H.-Y.; Ma Y.-Y.; Li Z.-J.; Li X.-M.; Li Y.-K.; Wang W.-G.; Zhou M.; Hu Q.-F.; Yang F.-X. Chem. Biol. Technol. Agric. 2023, 10, 138.
[6]	Nan Z.-D.; Shang Y.; Zhu Y.-D.; Zhang H.; Sun R.-R.; Tian J.-J.; Jiang Z.-B.; Ma X.-L.; Bai C. Phytochemistry 2025, 235, 114480.
[7]	Shabir G.; Saeed A.; Haq M.; Choudry F.; Kiran R.; Irfan M.; Tariq S.; El-Seedi H. R. Russ. J. Bioorg. Chem. 2024, 50, 2426.
[8]	Shabir G.; Saeed A.; El-Seedi H. R. Phytochemistry 2021, 181, 112568.
[9]	Noor A. O.; Almasri D. M.; Bagalagel A. A.; Abdallah H. M.; Mohamed S. G. A.; Mohamed G. A.; Ibrahim S. R. M. Molecules 2020, 25, 395.
[10]	Wang P.; Wang H.; Yang J.; Yang L.; Cai C.; Yuan J.; Wu F.; Gai C.; Mei W.; Dai H. Mar. Drugs 2023, 21, 150.
[11]	Yao L.; Jumai A.; Huang X.; Lin T.; Tao Z.; Qiu S.-X. RSC Adv. 2024, 14, 7763.
[12]	Bian H.; Ding B.; Huang J.; Huang R.; Liu J.; Peng H.; Wu K.; Chen Q.; Wang J.; Tao Y.; Huang H. Fitoterapia 2025, 183, 106555.
[13]	Wang C.; Xu H.; Wang J.; Wei C.; Zheng S.; Xu R.; Wang S.; Li Z.; Li P.; Kong F. J. Nat. Prod. 2025, 88, 757.
[14]	Tang Q.; Guo K.; Li X.-Y.; Zheng X.-Y.; Kong X.-J.; Zheng Z.-H.; Xu Q.-Y.; Deng X. Mar. Drugs 2014, 12, 5993.
[15]	Zhang Y.; Luo L.; Zhu S.; Niu S.; Zhang Y.; Zhang Y. RSC Adv. 2023, 13, 21954.
[16]	Liu C.; Li J.; Wang D.; Liu J.; Liu K.; Li P.; Zhang Y. Mar. Drugs 2024, 22, 540.

海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究

Study on Secondary Metabolites of Marine-Derived Fungus Cladosporium sp. MDCW-211

RichHTML

PDF

补充材料

可视化

摘要/Abstract

引用此文

分享此文

图/表 4

参考文献 16

相关文章 5

编辑推荐

相关统计

本文评价

[1]	易继凌, 施康琦, 吴冰林, 黎婉珊, 陈光英. 海洋来源真菌Eutypella sp. F0219次级代谢产物研究[J]. 有机化学, 2023, 43(1): 295-298.
[2]	张捷, 陈玉婵, 刘昭明, 郭波红, 高晓霞, 刘洪新, 章卫民. 海洋真菌赤褐炭团菌FS521的次级代谢产物研究[J]. 有机化学, 2020, 40(5): 1367-1371.
[3]	陈玲玲, 朱统汉, 朱国良, 刘云龙, 王聪, Pawinee Piyachaturawat, Arthit Chairoungdua, 朱伟明. 海洋来源真菌Penicillium brevicompactum OUCMDZ-4920的活性产物研究[J]. 有机化学, 2017, 37(10): 2752-2762.
[4]	庞冀燕 ; 刘岚; 佘志刚; 蔡小玲; 林永成*. 三类南海海洋真菌次级代谢产物的合成研究进展[J]. 有机化学, 2009, 29(03): 341-349.
[5]	朱峰, 林永成, 周世宁. 南海海洋真菌#2526中蒽醌类代谢产物的研究[J]. 有机化学, 2004, 24(9): 1114-1117.