海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究

doi:10.6023/cjoc202507017

研究简报

海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究

王见见^a, 郑圣䶮^a, 张洁^a, 高文鹏^a, 孔凡栋^a, 李培海^b, 王聪^a,*, 曹树更^c,*

^a广西民族大学化学化工学院林产化学与工程国家民委重点实验室广西林产化学与工程重点实验室林木生物质低碳高质利用广西高校工程研究中心广西民族大学南宁 530006;
^b山东省斑马鱼人类疾病模型与药物筛选工程技术研究中心齐鲁工业大学(山东省科学院)生物研究所济南 250103;
^c夏威夷大学药学院夏威夷 96720

收稿日期:2025-07-14 修回日期:2025-08-23
基金资助:
国家自然科学基金青年项目(82204276); 广西自然科学基金(No.2025GXNSFAA069557); 广西高等学校千名中青年骨干教师培育计划; 广西民族大学研究生教育创新计划项目(No.gxmzu-chxs2024225).

Study on Secondary Metabolites of Marine-derived Fungus Cladosporium sp. MDCW-211

Wang Jianjian^a, Zheng Shengyan^a, Zhang Jie^a, Gao Wenpeng^a, Kong Fandong^a, Li Peihai^b, Wang Cong^a,*, Cao Shugeng^c,*

^aKey Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission; Guangxi Key Laboratory of Chemistry and Engineering of Forest Products; Engineering Research Center of Low-carbon and High-quality Utilization of Forest Biomass, University of Guangxi; School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning, 530006, China;
^bEngineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China;
^cDepartment of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawai'i at Hilo, Hilo, HI 96720, USA

Received:2025-07-14 Revised:2025-08-23
Contact: *E-mail: wangcong123206@163.com; scao@hawaii.edu
Supported by:
National Natural Science Foundation of China (No. 82204276), the Guangxi Natural Science Foundation (No.2025GXNSFAA069557), the Guangxi Scholarship Fund of Guangxi Education Department, and Innovation Project of Guangxi Minzu University Graduate Education (No.gxmzu-chxs2024225) ).

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摘要/Abstract

对北部湾涠洲岛来源的海洋真菌Cladosporium sp. MDCW-211产生的次级代谢产物及其生物活性进行研究。利用制备型高效液相色谱等技术进行化合物的分离纯化,运用HR-ESI-MS、NMR、X-ray等方法对化合物的结构进行鉴定,采用斑马鱼模型评估化合物的促血管生成活性。分离鉴定了1个新的二氢异香豆素类化合物1（4'S-hydroxyasperentin）以及3个已知二氢异香豆素类化合物2-4。活性结果表明,化合物3在浓度为20和40 μmol/L时具有促血管生成活性。化合物1为新的4'位为α-OH的二氢异香豆素类化合物,首次报道了化合物3的促血管生成活性。

关键词: 海洋真菌, Cladosporium sp. MDCW-211, 二氢异香豆素类化合物, 促血管生成活性

A study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp. MDCW-211. Preparative High Performance Liquid Chromatography (HPLC) and other techniques were used to isolate and purify the compounds, the structures of the compounds were determined by HR-ESI-MS, NMR, X-ray and other methods, and the pro-angiogenic activity of the compounds was evaluated by zebrafish model. One new dihydroisocoumarin 1 (4'S-hydroxyasperentin) and three known dihydroisocoumarins 2-4 were obtained and identified. Compound 3 was tested at 20 and 40 μmol/L, and it demonstrated strong pro-angiogenic activity, which is reported here for the first time.

Key words: Marine fungus, Cladosporium sp. MDCW-211, dihydroisocoumarins, pro-angiogenic activity

引用此文

王见见, 郑圣䶮, 张洁, 高文鹏, 孔凡栋, 李培海, 王聪, 曹树更. 海洋来源真菌Cladosporium sp. MDCW-211次级代谢产物研究[J]. 有机化学, doi: 10.6023/cjoc202507017.

Wang Jianjian, Zheng Shengyan, Zhang Jie, Gao Wenpeng, Kong Fandong, Li Peihai, Wang Cong, Cao Shugeng. Study on Secondary Metabolites of Marine-derived Fungus Cladosporium sp. MDCW-211[J]. Chinese Journal of Organic Chemistry, doi: 10.6023/cjoc202507017.

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