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有机化学
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有机化学 ›› 2003, Vol. 23 ›› Issue (11): 1260-1263. 上一篇    下一篇

研究论文

有机相中α-氰基-3-苯氧基苄醇乙酯的酶促醇解反应

杨立荣;罗积杏;吴坚平;唐世平   

  1. 浙江大学材料与化工学院生物工程研究所
  • 发布日期:2003-11-25

Lipase-Catalyzed Alcoholysis of α-Cyano-3-phenoxybenzyl Acetate in Organic Media

Yang Lirong;Luo Jixing;Wu Jianping;Tang Shiping   

  1. Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University
  • Published:2003-11-25

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被引次数

10 | 2

研究了有机相中脂肪酶催化α-氰基-3-苯氧基苄醇乙酯的醇解化反应。制备α -氰基-3-苯氧基苄醇。考察了酶、溶剂、醇、醇用量、溶剂水含量以及底物浓度等 因素对反应的影响，结果表明Novozym435脂肪酶催化活性最高，经实验确定的最佳 条件为：脱水甲苯为溶剂，正辛醇为酰基受体，正辛醇、酯的摩尔比为1.5:1，酶 量为8 mg/mL时的最佳底物浓度为108.13 mmol/L，在上述条件下反应30 h酯的转化 率 > 96%。

关键词: 脂肪酶, 醇解, 苯氧基苄醇乙酯, 正辛醇, 催化活性

The lipase-catalyzed alcoholysis of α-cyano-3-phenoxybenzyl acetate (CPBAc) in organic solvents for the synthesis of α-cyano-3- phenoxybenzyl alcohol (CPBA) was studied. The experimental results indicated that Novozym435 lipase showed the highest catalytic activity for the reaction. 1-Octanol was founed to be the best acyl acceptor. Toluene was the preferred solvent and the optimum molar ratio of 1- octanol to CPBAc was about 1.5 : 1. The reaction rate was slowed down with the addition of water to the solvent. The best concentration of substrate was 108.13 mmol/L under 8 mg/mL lipase for the reaction. When the reaction was carried out under the above optimum conditions, more than 96% of conversion was achieved.

Key words: LIPASE;ALCOHOLYSIS;phenoxybenzyl acetate;N-OCTYL ALCOHOL, CATALYTIC ACTIVITY

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