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有机化学
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有机化学 ›› 2005, Vol. 25 ›› Issue (06): 629-633. 上一篇    下一篇

综述与进展

醇脱氢酶结构和作用机理研究进展

许松伟，姜忠义*，吴洪   

  1. (天津大学化工学院 天津 300072)
  • 收稿日期:2004-05-24 修回日期:2004-11-18 发布日期:2005-05-30
  • 通讯作者: 姜忠义

Progress in Structure and Kinetic Mechanism of Alcohol Dehydro-genase

XU Song-Wei, JIANG Zhong-Yi*, WU Hong   

  1. (School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072)
  • Received:2004-05-24 Revised:2004-11-18 Published:2005-05-30

PDF

4252

被引次数

22

介绍了醇脱氢酶的种类, 酵母醇脱氢酶和肝醇脱氢酶等两类常用的醇脱氢酶的物理化学性质和活性位点结构. 归纳了对肝醇脱氢酶和酵母醇脱氢酶作用机理的研究, 重点评述了醇脱氢酶催化反应中的两个关键步骤质子转移和氢化物转移过程机理的研究进展.

关键词: 机理, 氢化物, 质子, 醇脱氢酶, 烟酰胺腺嘌呤二核苷酸

The type of alcohol dehydrogenases, the chemical and physical properties and structure of active site of two common types of alcohol dehydrogenase, yeast alcohol dehydrogenase (YADH) and liver alcohol dehydrogenase (LADH) were introduced. Research progress in the kinetic mechanism of YADH and LADH was summarized with special focus on the progress in the proton transfer and hydride transfer, two key steps involved in the dehydrogenase-catalyzed reaction.

Key words: hydride, nicotinamide adenine dinucleotide, proton, mechanism, alcohol dehydrogenase