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有机化学
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有机化学 ›› 2008, Vol. 28 ›› Issue (05): 861-864. 上一篇    下一篇

研究简报

柔荑软珊瑚Nephthea sp.中两种具有细胞毒活性的甾醇化合物

徐石海*， 廖小建，林慧   

  1. (暨南大学化学系 广州 510632 )
  • 收稿日期:2007-08-07 修回日期:2007-10-06 发布日期:2008-05-20
  • 通讯作者: 徐石海

Two Cytotoxic Sterols from the Soft Coral Nephthea sp.

XU Shi-Hai*, LIAO Xiao-Jian, LIN Hui   

  1. (Department of Chemistry, Jinan University, Guangzhou 510632)
  • Received:2007-08-07 Revised:2007-10-06 Published:2008-05-20
  • Contact: XU Shi-Hai

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采用抗肿瘤活性追踪法对采自中国南海西沙群岛永兴岛柔荑软珊瑚Nephthea sp.中抗肿瘤活性成分进行研究, 报道了两种具有细胞毒活性的甾醇化合物, 经过MS, IR, 1H NMR, 13C NMR (DEPT), HSQC 和HMBC等光谱技术鉴定为: 24-甲基胆甾-5-烯-3β,8β-二醇(1)和24-亚甲基胆甾-4-烯-3β,6β-二醇(2), 化合物1为新化合物, 利用X-ray单晶衍射技术验证化合物2的立体结构. 化合物1对肝癌细胞(BEL-7402), 小鼠白血瘤细胞(P388)和人结肠癌细胞(LOVO)作用的IC50值分别为18.0, 25.5和20.6 μg/mL; 化合物2对肿瘤细胞P388和人乳腺癌细胞(MCF)作用的IC50值分别为1.0和2.5 μg/mL.

关键词: 软珊瑚, Nephthea sp, 甾醇, 抗肿瘤活性

Two cytotoxic sterols have been isolated from Nephthea sp. collected from Xisha islands of the South China Sea by bioassay-guided isolation. Their structures were established as 24-methylcholestan-5- ene-3β,8β-diol (1) and 24-methylenecholestan-4-ene-3β,6β-diol (2) by MS, IR, 1H NMR, 13C NMR (DEPT), HSQC and HMBC analysis. Sterol 1 is a previously unknown compound and the stereochemistry of sterol 2 has been validated by X-ray diffraction analysis for the first time. Sterol 1 exhibited cytotoxicity against BEL-7402, P388 and LOVO cells (IC50 18.0, 25.5 and 20.6 μg/mL, respectively). Sterol 2 also showed significant cytotoxicity against P388 and MCF cells (IC50 1.0 and 2.5 μg/mL, respectively).

Key words: Nephthea sp., sterol, cytotoxicity, coral