关键词: 快原子轰击质谱, 氨基酸顺序分析, 分子量, 天花粉蛋白, 质谱法, 蛋白质, 串联质谱, 多肽, 糖肽, 结构分析

This article introduces four newly developed mass spectrometric methods. There are fast atom bombardment-MS, (FAB-MS), tandem mass spectrometry (MS/MS), electrospray ionization-MS(ESI-MS) and matrix assisted laser desorption ionization time of flight-MS(MALDI-TOF-MS). These developments make MS be used for the structural analysis of protein. This paper also discusses their application in the following aspects by several examples: the molecular weight determination of proteins, appraise of the purity of proteins or peptides, the structural determination of a glycopeptide and the structural determination of the N-blocked or normal peptides and proteins.

Key words: FAB MASS SPECTROMETRY, MASS SPECTROGRAPHY, AMINO ACID SEQUENCE ANALYSIS, RICHOSATHIN PROTEIN, GLYCOPEPTIDE, POLYPEPTIDE, PROTEIN, STRUCTURAL ANALYSIS, MOLECULAR WEIGHT