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有机化学
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有机化学 ›› 2016, Vol. 36 ›› Issue (4): 867-871.DOI: 10.6023/cjoc201511023 上一篇    下一篇

研究简报

利用聚甲基乙烯基醚共聚马来酸银簇实现高半胱氨酸探测

杨太群a,b, 戴山a, 秦翠芳a, 黄科翰a, 陈瑜婷a, 潘海峰a, 张三军a,c, 张坤b, 徐建华a,c   

  1. a. 华东师范大学 精密光谱科学与技术国家重点实验室 上海 200062;
    b. 华东师范大学化学系 上海市绿色化学与化工过程绿色化重点实验室 上海 200062;
    c. 山西大学极端光学协同创新中心 太原 030006
  • 收稿日期:2015-11-13 修回日期:2015-12-03 发布日期:2015-12-10
  • 通讯作者: 张三军 E-mail:sjzhang@phy.ecnu.edu.cn
  • 基金资助:

    上海市自然科学基金(No.15ZR1410100)、国家自然科学基金(No.61178085)资助项目.

Selective Detection of Homocysteine by Using Poly Methyl Vinyl Ether-alt-Maleic Silver Clusters

Yang Taiquna,b, Dai Shana, Qin Cuifanga, Huang Kehana, Chen Yutinga, Pan Haifenga, Zhang Sanjuna,c, Zhang Kunb, Xu Jianhuaa,c   

  1. a. State Key Laboratory of Precision Spectroscopy, East China. Normal University, Shanghai 200062;
    b. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry East China. Normal University, Shanghai 200062;
    c. Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006
  • Received:2015-11-13 Revised:2015-12-03 Published:2015-12-10
  • Supported by:

    Project supported by the Science and Technology Commission of Shanghai Municipality (No. 15ZR1410100) and the National Natural Science Fundation of China (No. 61178085).

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报道采用聚甲基乙烯基醚共聚马来酸为模板合成的发光银簇实现高半胱氨酸选择性探测,该银簇在540和460nm处具有两个发射峰.在高半胱氨酸加入之后,高半胱氨酸的巯基通过配位作用与银簇结合,造成其在540和460nm处的发光强度降低,两个发光峰强度的比值随高半胱氨酸的浓度的增加呈线性变化.该探针的检测限为1μmol/L,线性响应范围为5~100μmol/L.该方法利用双峰比值的变化来检测高半胱氨酸,有很高的选择性,可以实现高半胱氨酸浓度的定量检测.

关键词: 高半胱氨酸, 聚甲基乙烯基醚共聚马来酸, 荧光探针, 银簇

We report selective detection of homocysteine (Hcy) through luminescent poly methyl vinyl ether-alt-maleic acid (PMVEM) silver nanoclusters (Ag NCs), which were synthesized using PMVEM as scaffold. The as-developed PMVEM-Ag NCs have two emission peaks at 460 and 540 nm. After Hcy was added, the thiol moiety of Hcy can efficiently coordinate on Ag NCs, resulting in luminescence quenching of the 460 and 540 nm peaks. However, the ratio of these two emission peak was linearly correlated with the Hcy concentration from 5 to 100 μmol/L. The detection limits was 1 μmol/L. The detection of Hcy used the ratio of the two emission peaks, which permit selective detection of Hcy and quantitive determination of Hcy levels.

Key words: homocysteine, poly methyl vinyl ether-alt-maleic acid, fluorescent sensor, silver nanoclusters