有机化学 ›› 2019, Vol. 39 ›› Issue (12): 3583-3587.DOI: 10.6023/cjoc201904066 上一篇    下一篇

研究简报

小连翘化学成分的研究

李竣a, 徐晓诗a, 滕海达b, 陈玉b, 梅之南a, 杨光忠a   

  1. a 中南民族大学药学院 武汉 430074;
    b 中南民族大学化学与材料科学学院 武汉 430074
  • 收稿日期:2019-04-26 修回日期:2019-06-17 发布日期:2019-07-17
  • 通讯作者: 梅之南, 杨光忠 E-mail:meizhinan@163.com;yanggz888@126.com
  • 基金资助:
    国家重大新药创制科技重大专项(No.2017Z09301060)和中央高校基本科研业务费(No.CZP18004)资助项目.

Chemical Constituents from Hypericum Erectum Thunb

Li Juna, Xu Xiaoshia, Teng Haidab, Chen Yub, Mei Zhinana, Yang Guangzhonga   

  1. a School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074;
    b College of Chemistry and Materials Sciences, South-Central University for Nationalities, Wuhan 430074
  • Received:2019-04-26 Revised:2019-06-17 Published:2019-07-17
  • Supported by:
    Project supported by the National Major New Drugs Innovation and Development (No. 2017ZX09301060) and the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (No. CZP18004).

采用溶剂提取法和正、反相硅胶柱色谱及高效液相等多种色谱技术对小连翘化学成分进行分离纯化,并根据理化性质和现代波谱技术对其进行结构鉴定.从其氯仿提取物分离3个结构类型分别属于吡喃酮、黄酮和脂肪醇的新化合物以及15个已知化合物.以脂多糖(LPS)诱导RAW264.7细胞建立炎症模型,采用Griess法测定NO的分泌量,其中hyperiflavone(2)、isocudraniaxan-thone B(5)和2-deprenylrheediaxanthone B(6)对NO分泌表现出一定的抑制活性,其IC50分别为12.90,16.24和9.86 μmol·L-1.

关键词: 金丝桃属, 小连翘, 呫吨酮, 抗炎活性

The chemical constituents of Hypericum erectum Thunb were isolated and purified by solvent extraction method, the normal and reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography. Their structures were elucidated by their physicochemical property and extensive analysis of spectroscopic data. Three new compounds which belong to α-pyranone, flavonoid and aliphatic alcohol respectively, together with 15 known compounds, were isolated from the chloroform extract of H. erectum. Inflammatory cell model was constructed by lipopolysaccharide (LPS)-stimulated RAW264.7 cell line. NO production inhibitory effects of all tested compounds were measured by Griess method. Hyperiflavone (2), isocudraniaxan-thone B (5) and 2-deprenylrheediaxanthone B (6) exhibited moderate NO production inhibitory activities with the IC50 values of 16.24, 9.86 and 12.90 μmol·L-1, respectively.

Key words: Hypericum Linn, Hypericum erectum Thunb, xanthone, anti-inflammatory activity