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有机化学
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有机化学 ›› 2020, Vol. 40 ›› Issue (1): 209-214.DOI: 10.6023/cjoc201907041 上一篇    下一篇

研究简报

巴戟天内生真菌Trichoderma spirale A725中两个新的聚酮类化合物

陈书帅a,b, 刘洪新b, 刘昭明b, 李赛妮b, 陈玉婵b, 李浩华b, 李冬利a, 章卫民b   

  1. a 五邑大学生物科技与大健康学院 广东江门 529020;
    b 广东省微生物研究所 广东省科学院 华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室 广东省微生物应用新技术公共实验室 广州 510075
  • 收稿日期:2019-07-26 修回日期:2019-09-10 发布日期:2019-09-25
  • 通讯作者: 章卫民, 李冬利 E-mail:wmzhang@gdim.cn;wyuchemldl@wyu.edu.cn
  • 基金资助:
    国家自然科学基金（No.31600271）、广东省科技计划（No.2107A020211023）和广州市珠江科技新星（No.201806010080）资助项目.

Two New Polyketide Compounds from the Endophytic Fungus Trichoderma spirale A725 of Morinda officinalis

Chen Shushuaia,b, Liu Hongxinb, Liu Zhaomingb, Li Sainib, Chen Yuchanb, Li Haohuab, Li Donglia, Zhang Weiminb   

  1. a School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, Guangdong 529020;
    b State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070
  • Received:2019-07-26 Revised:2019-09-10 Published:2019-09-25
  • Supported by:
    Project supported by the National Natural Science Foundation of China (No. 31600271), the Guangdong Provincial Project for Science and Technology (No. 2107A020211023), and the Pearl River Science and Technology New Star Fund of Guangzhou City (No. 201806010080).

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补充材料

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综合运用硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱技术对药用植物巴戟天内生真菌Trichoderma spirale A725的次生代谢产物进行分离纯化，得到6个聚酮类化合物，采用多种现代波谱技术确定其结构，分别为：6-羟基-4-异丙基-1，8-二甲基螺环[4.5]癸-1，8-二烯-7-酮（1），2-羟基-2，5-二甲基-7-氧代-5，7-二氢-2H-呋喃[3，4-b]吡喃-4-羧酸（2），3-乙基-4-羟基-6-甲基-二氢-吡喃-2-酮（3），苯乙内酯A（4），3-羟基-5-（4-羟基苄基）二氢呋喃-2（3H）-酮（5），4-乙酰-3-羟基-6-甲基吡喃-2-酮（6）.其中化合物1和2为新化合物，化合物3为新天然产物.此外，利用四株肿瘤细胞株（HepG-2、MCF-7、SF-268及A549）对化合物1~6细胞毒活性进行评估，结果表明化合物1~6对上述肿瘤细胞均无明显的细胞毒活性.

关键词: 巴戟天, 内生真菌, 螺旋木霉, 聚酮

Six polyketides were isolated from the fermentation broth of an endophytic fungus Trichoderma spirale A725 of the medicinal plant Morinda officinalis by silica gel, reversed phase silica column chromatography, sephadex LH-20 and high performance preparative liquid chromatography. Their structures were elucidated to be 6-hydroxy-4-isopropyl-1,8-dimethyl-spiro[4.5]deca-1,8-dien-7-one (1), 2-hydroxy-2,5-dimethyl-7-oxo-5,7-dihydro-2H-furo[3,4-b]pyran-4-carboxylicacid (2), 3-ethyl-4-hydroxy-6-methyl-2H-pyran-2-one (3), harzialactone A (4), 3-hydroxy-5-(4-hydroxybenzyl)dihydrofuran-2(3H)-one (5), and 4-acetyl-3-hydroxy-6-methyl-pyran-2-one (6) on the basis of extensive spectral analysis. Among them, compounds 1 and 2 were new compounds, and compound 3 was a new natural product. Moreover, the cytotoxicities of compounds 1~6 were evaluated against four cancer cell lines (Hep G-2, MCF-7, SF-268 and A549). However, none of them exhibited cytotoxic activity against the tested tumor cells.

Key words: Morinda officinalis, endophyte fungus, Trichoderma spirale, polyketide