A Hg2+-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

doi:10.6023/cjoc201708061

Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (4): 926-930.DOI: 10.6023/cjoc201708061 Previous Articles Next Articles

Special Issue: 荧光探针-生物传感合辑；有机超分子化学合辑

Articles

一种Hg²⁺荧光探针的合成及其在活细胞成像中的应用

张敏^a, 肖慧丰^a, 韩志湘^b, 仰榴青^a, 吴向阳^b

a 江苏大学化学化工学院镇江 212013;
b 江苏大学环境与安全工程学院镇江 212013

收稿日期:2017-08-30 修回日期:2017-11-09 发布日期:2017-12-01
通讯作者: 吴向阳 E-mail:wuxy@ujs.edu.cn
基金资助:
江苏省自然科学基金（No.BK20140536）资助项目.

A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

Zhang Min^a, Xiao Huifeng^a, Han Zhixiang^b, Yang Liuqing^a, Wu Xiangyang^b

a School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013;
b School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013

Received:2017-08-30 Revised:2017-11-09 Published:2017-12-01
Contact: 10.6023/cjoc201708061 E-mail:wuxy@ujs.edu.cn
Supported by:
Project supported by the Natural Science Foundation of Jiangsu Province (No. BK20140536).

1. A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging.PDF(414KB)

Abstract

A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by ¹H NMR, ¹³C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg²⁺ ions were investigated in ethanol-water (V/V=7/3, HEPES 10 mmol/L, pH=7.0) by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable "turn-on" fluorescent responses to Hg²⁺ with good selectivity over the other metal ions examined. The 1:1 binding model of probe 1 with Hg²⁺ was established by Job's plot and MS spectra. The probe can be applied to the quantitative analysis of Hg²⁺ with a linear range covering from 0 to 50 μmol/L. The fluorescence imaging experiments in MGC-803 living cells demonstrated that 1 could be used in biological systems to monitor Hg²⁺.

Key words: rhodamine B, synthesis, fluorescent probe, Hg²⁺, bioimaging

Cite this article

Zhang Min, Xiao Huifeng, Han Zhixiang, Yang Liuqing, Wu Xiangyang. A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging[J]. Chin. J. Org. Chem., 2018, 38(4): 926-930.

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一种Hg²⁺荧光探针的合成及其在活细胞成像中的应用

A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

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一种Hg2+荧光探针的合成及其在活细胞成像中的应用